| Title: | Effect of inoculation process on lycopene production by Blakeslea trispora in a stirred-tank reactor |
| Author(s): | Wang Q; Feng LR; Luo W; Li HG; Zhou Y; Yu XB; |
| Address: | "The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, China" |
| DOI: | 10.1007/s12010-014-1327-y |
| ISSN/ISBN: | 1559-0291 (Electronic) 0273-2289 (Linking) |
| Abstract: | "Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (-) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/-) at the 36 and 48 h inoculum age of mating types (+) and (-), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (-) mating type. Morphological observation showed that the mycelium ratio of B. trispora (-) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (-) in joint cultivation and hence stimulated lycopene biosynthesis" |
| Keywords: | Biomass Bioreactors Carotenoids/*biosynthesis Fermentation Glucose/metabolism Hydrogen-Ion Concentration Lycopene Mating Factor Mucorales/*metabolism Mycelium/*metabolism Peptides/*physiology Time Factors; |
| Notes: | "MedlineWang, Qiang Feng, Ling-Ran Luo, Wei Li, Han-Guang Zhou, Ya Yu, Xiao-Bin eng Research Support, Non-U.S. Gov't 2014/10/25 Appl Biochem Biotechnol. 2015 Jan; 175(2):770-9. doi: 10.1007/s12010-014-1327-y. Epub 2014 Oct 24" |
