| Title: | Characterization of Volatile Organic Compounds Produced by Bacillus siamensis YJ15 and Their Antifungal Activity Against Botrytis cinerea |
| Author(s): | Wang C; Duan T; Shi L; Zhang X; Fan W; Wang M; Wang J; Ren L; Zhao X; Wang Y; |
| Address: | "College of Plant Protection, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. China. State Key Laboratory of Sustainable Dryland Agriculture (in preparation), Shanxi Agricultural University, Taiyuan, Shanxi 030031, P.R. China. Experiment Teaching Center, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. China" |
| DOI: | 10.1094/PDIS-01-22-0230-RE |
| ISSN/ISBN: | 0191-2917 (Print) 0191-2917 (Linking) |
| Abstract: | "To develop an effective and environmentally safe strategy to control postharvest gray mold caused by Botrytis cinerea, Bacillus siamensis strain YJ15 isolated from blueberry was used to test the biocontrol potential. It is interesting to find that the volatile organic compounds (VOCs) emitted from strain YJ15 exhibited significant antifungal activity against Botrytis cinerea as well as 11 other plant-pathogenic fungi, with a percentage of mycelial growth inhibition from 74.96 to 92.81%. Additionally, VOCs from strain YJ15 could reduce significantly the disease incidence and lesion diameter with the increasing of fermentation time, indicating great biocontrol potential for controlling blueberry postharvest gray mold. Furthermore, the VOCs were collected by using headspace solid-phase microextraction fiber, and the composition of VOCs was further revealed by using gas chromatography coupled with quadruple mass spectrometry. In total, 24 kinds of VOCs, including 5 alkanes, 2 aldehydes, 3 ketones, 5 alcohols, 1 alkene, 5 acids and esters, 2 aromatic compounds, and 1 sulfur compound, were emitted at 1, 3, 5, and 7 days after inoculation. Among these VOCs, eight antifungal VOCs could inhibit mycelial growth of B. cinerea. It is important to highlight that, although 1-butanol and 3-methyl-1-butanol were the most abundant compounds, 2-ethylhexanol, 1-heptanol, and 1,3-xylene have proved to be more toxic to B. cinerea than 3-methyl-1-butanol, propanethioic acid, 2,2-dimethyl-, ethyl 2-methylbutyrate, 2-heptanone, and 1-butanol, which provide new, promising biofumigants for the control of postharvest gray mold caused by B. cinerea" |
| Keywords: | 1-Butanol/pharmacology Antifungal Agents/pharmacology Bacillus Botrytis Fruit/microbiology *Volatile Organic Compounds/pharmacology Bacillus siamensis Botrytis cinerea antifungal activity cultural and biological practices disease management fruit fungi po; |
| Notes: | "MedlineWang, Chunwei Duan, Tiankun Shi, Luxin Zhang, Xiqian Fan, Weixin Wang, Meiqin Wang, Jianming Ren, Lu Zhao, Xiaojun Wang, Yan eng 2022/04/06 Plant Dis. 2022 Sep; 106(9):2321-2329. doi: 10.1094/PDIS-01-22-0230-RE. Epub 2022 Aug 10" |
