| Title: | Noninvasive detection of colorectal cancer by analysis of exhaled breath |
| Author(s): | Wang C; Ke C; Wang X; Chi C; Guo L; Luo S; Guo Z; Xu G; Zhang F; Li E; |
| Address: | "Department of Anesthesiology, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng St, Nangang District, 150001, Harbin, China" |
| DOI: | 10.1007/s00216-014-7865-x |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "There has been growing interest in exhaled breath analysis for cancer screening and disease monitoring; however, limited breath biomarker information exists regarding colorectal cancer (CRC). The objective of this study was to screen for breath biomarkers of CRC. Exhaled breath was collected from 20 CRC patients and 20 healthy controls; subsequently, solid-phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to assess the exhaled volatile organic compounds (VOCs) of the study participants. The statistical methods of principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to process the final data. The VOCs in the exhalations of CRC patients exhibited significant differences from the VOCs in the exhalations of healthy controls; in particular, relative to the latter exhalations, the former exhalations contain significantly higher levels of cyclohexanone, 2,2-dimethyldecane, dodecane, 4-ethyl-1-octyn-3-ol, ethylaniline, cyclooctylmethanol, trans-2-dodecen-1-ol, and 3-hydroxy-2,4,4-trimethylpentyl 2-methylpropanoate but significantly lower levels of 6-t-butyl-2,2,9,9-tetramethyl-3,5-decadien-7-yne (P < 0.05). Analyses of breath VOCs provide a related model of CRC exhalation that could represent an effective and convenient screening method for this disease" |
| Keywords: | Breath Tests/*methods Colorectal Neoplasms/*diagnosis Discriminant Analysis Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Middle Aged Principal Component Analysis Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineWang, Changsong Ke, Chaofu Wang, Xiaoyang Chi, Chunjie Guo, Lei Luo, Suqi Guo, Zhigang Xu, Guowang Zhang, Fengmin Li, Enyou eng Research Support, Non-U.S. Gov't Germany 2014/05/14 Anal Bioanal Chem. 2014 Jul; 406(19):4757-63. doi: 10.1007/s00216-014-7865-x. Epub 2014 May 13" |
