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Pest Manag Sci

Title:		"Isolation and identification of floral attractants from a nectar plant for the dried bean beetle, Acanthoscelides obtectus (Coleoptera: Chrysomelidae, Bruchinae)"
Author(s):		Vuts J; Woodcock CM; Caulfield JC; Powers SJ; Pickett JA; Birkett MA;
Address:		"Rothamsted Research, Harpenden, UK"
Journal Title:		Pest Manag Sci
Year:		2018
Volume:		20180308
Issue:
Page Number:		-
DOI:		10.1002/ps.4903
ISSN/ISBN:		1526-4998 (Electronic) 1526-498X (Linking)
Abstract:		"BACKGROUND: The response of virgin females of the legume pest Acanthoscelides obtectus (Coleoptera: Bruchidae) to headspace extracts of volatiles collected from flowers of a nectar plant, Daucus carota, was investigated using behaviour (four-arm olfactometry) and coupled gas chromatography-electroantennography (GC-EAG). RESULTS: Odours from inflorescences were significantly more attractive to virgin female beetles than clean air. Similarly, a sample of volatile organic compounds (VOCs) collected by air entrainment (dynamic headspace collection) was more attractive to beetles than a solvent control. In coupled GC-EAG experiments with beetle antennae and the VOC extract, six components showed EAG activity. Using coupled GC-mass spectrometry (GC-MS) and GC peak enhancement with authentic standards, the components were identified as alpha-pinene (S:R 16:1), sabinene, myrcene, limonene (S:R 1:3), terpinolene and (S)-bornyl acetate. Females preferred the synthetic blend of D. carota EAG-active volatiles to the solvent control in bioassays. When compared directly, odours of D. carota inflorescences elicited stronger positive behaviour than the synthetic blend. CONCLUSION: This is the first report of behaviourally active volatiles linked to pollen location for A. obtectus, and development of the six-component blend is being pursued, which could underpin the design of semiochemical-based field management approaches against this major pest of stored products. (c) 2018 Society of Chemical Industry"
Keywords:		Acanthoscelides obtectus attractant behavioural assays chemical ecology gas chromatography-electroantennography (GC-EAG) plant volatiles;
Notes:		"PublisherVuts, Jozsef Woodcock, Christine M Caulfield, John C Powers, Stephen J Pickett, John A Birkett, Michael A eng England 2018/03/09 Pest Manag Sci. 2018 Mar 8. doi: 10.1002/ps.4903"