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Anim Genet

Title:		cDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4 isomerase
Author(s):		von Teichman A; Joerg H; Werner P; Brenig B; Stranzinger G;
Address:		"Institute of Animal Science, Swiss Federal Institute of Technology, ETH Zentrum, Zurich, Switzerland"
Journal Title:		Anim Genet
Year:		2001
Volume:		32
Issue:		5
Page Number:		298 - 302
DOI:		10.1046/j.1365-2052.2001.00775.x
ISSN/ISBN:		0268-9146 (Print) 0268-9146 (Linking)
Abstract:		"The 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4-isomerase (3 beta-HSD) enzymes are essential for the biosynthesis of steroid hormones. The 3 beta-HSD gene family has been reported to encode for different isoenzymes which function either as dehydrogenase/isomerase or as reductase. The 3 beta-HSD enzymes are involved in the formation of the pheromone androstenone (5 alpha-androst-16-ene-3-one) which contributes to the unpleasant odour present in the meat of uncastrated boars. An reverse-transcription-polymerase chain reaction (RT-PCR) probe from porcine testicular tissue of a 3 beta-HSD enzyme was used to screen a porcine adipose tissue cDNA library. Both strands of the positive clones were sequenced and the putative coding sequence of 1122 nucleotides encodes 374 amino acids. Comparison of the putative open reading frame with the bovine and the human type I homologues revealed 85.6 and 79.3% identity, respectively. Fluorescence in situ hybridization (FISH) was performed with a labelled PAC clone containing the gene of interest. The 3 beta-HSD gene was mapped to the porcine chromosome 4q16-4q21 which is in accordance with the comparative gene map"
Keywords:		"Amino Acid Sequence Animals Cloning, Molecular DNA Primers DNA, Complementary Humans Male Metaphase Molecular Sequence Data Multienzyme Complexes/*genetics *Physical Chromosome Mapping/veterinary Progesterone Reductase/*genetics Sequence Homology, Amino A;"
Notes:		"Medlinevon Teichman, A Joerg, H Werner, P Brenig, B Stranzinger, G eng Research Support, Non-U.S. Gov't England 2001/10/31 Anim Genet. 2001 Oct; 32(5):298-302. doi: 10.1046/j.1365-2052.2001.00775.x"