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Cold Spring Harb Protoc


Title:Female Fly Postmating Behaviors
Author(s):von Philipsborn AC; Shohat-Ophir G; Rezaval C;
Address:"Danish Research Institute of Translational Neuroscience (DANDRITE), Nordic-EMBL Partnership for Molecular Medicine, Aarhus University, 8000 Aarhus, Denmark. The Mina and Everard Goodman Faculty of Life Sciences, The Leslie and Susan Gonda Multidisciplinary Brain Research Center, the Nanotechnology Institute, Bar-Ilan University, Ramat Gan 5290002, Israel galit.ophir@biu.ac.il c.rezaval@bham.ac.uk. School of Biosciences, University of Birmingham, Birmingham B15 2TT, United Kingdom galit.ophir@biu.ac.il c.rezaval@bham.ac.uk"
Journal Title:Cold Spring Harb Protoc
Year:2023
Volume:20230705
Issue:7
Page Number:db -
DOI: 10.1101/pdb.prot108108
ISSN/ISBN:1559-6095 (Electronic) 1559-6095 (Linking)
Abstract:"Upon copulation, females undergo a switch-like change in their behavior and physiology, known as 'postmating responses.' These strong behavioral and physiological changes are triggered by the transfer of male seminal proteins during copulation. Postmating response is associated with strong reduction in receptivity, indicated by the females kicking their legs toward the suitor and curving their abdomen downward to hide their genitalia from them and extruding their ovipositor at the tip of the abdomen, which physically prevents copulation. The transfer of male-specific pheromones, such as 11-cis-vaccenyl-acetate, during copulation further reduces female attractiveness. In addition, mated females exhibit increased ovulation, egg-laying behavior, enhanced feeding behavior, and changes in food preference. However, females increase their rate of remating when they are in social groups or in the presence of food. This protocol describes methods for measuring female postmating behaviors, such as oviposition, female sexual receptivity, and mating plug ejection"
Keywords:"Animals Female Male *Sexual Behavior, Animal/physiology *Copulation Ovulation;neuroscience;"
Notes:"Medlinevon Philipsborn, Anne C Shohat-Ophir, Galit Rezaval, Carolina eng Research Support, Non-U.S. Gov't 2023/02/14 Cold Spring Harb Protoc. 2023 Jul 5; 2023(7):.pdb.prot108108. doi: 10.1101/pdb.prot108108"

 
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