| Title: | The elusive crypt olfactory receptor neuron: evidence for its stimulation by amino acids and cAMP pathway agonists |
| Author(s): | Vielma A; Ardiles A; Delgado L; Schmachtenberg O; |
| Address: | "Centro de Neurociencia de Valparaiso, Universidad de Valparaiso, Avda. Gran Bretana 1111, Casilla 5029, Correo 4 2360102 Valparaiso, Chile" |
| ISSN/ISBN: | 0022-0949 (Print) 0022-0949 (Linking) |
| Abstract: | "Crypt olfactory receptor neurons (ORNs) are a third type of chemosensory neuron along with ciliated and microvillous ORNs in the olfactory epithelium of fishes, but their functional role is still unknown. To investigate their odorant response properties and possible transduction pathways, we recorded crypt ORN activity with calcium imaging and the patch clamp technique in its cell-attached mode in combination with odorant and agonist stimulation. Bile salts and putative fish pheromones did not elicit responses with either method, but the cells frequently responded to amino acids, with excitation and intracellular calcium signals. 8Br-cAMP and IBMX plus forskolin stimulated over 40% of crypt ORNs and triggered calcium signals in a similar percentage. Furthermore, crypt ORNs were immunoreactive to an antiserum against adenylate cyclase III. Together, these data suggest the presence of a cAMP transduction pathway, which might transduce odorants such as amino acids" |
| Keywords: | "Adenylyl Cyclases/metabolism Amino Acids/*pharmacology Animals Blotting, Western Calcium/metabolism Cyclic AMP/agonists/*pharmacology Fishes/*metabolism Immunohistochemistry Membrane Potentials/drug effects Olfactory Receptor Neurons/cytology/*drug effect;" |
| Notes: | "MedlineVielma, Alex Ardiles, Alvaro Delgado, Luz Schmachtenberg, Oliver eng Research Support, Non-U.S. Gov't England 2008/07/16 J Exp Biol. 2008 Aug; 211(Pt 15):2417-22. doi: 10.1242/jeb.018796" |
