| Title: | ClNOX1/ClNOXR-mediated MAPK and cAMP-PKA signalling pathways and ROS metabolism are involved in Curvularia lunata sexual reproduction and host infection |
| Author(s): | Wang F; Liu K; Wang J; Sun Y; Xiao S; Xue C; |
| Address: | "College of Plant Protection, Shenyang Agriculture University, Shenyang, 110161, China" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "NADPH oxidases (NOXs) and hydrogen peroxide (H(2) O(2) ) are involved in physiological and pathological processes, and cell fate decisions in organisms. However, regulatory mechanism of NOXs and the role of H(2) O(2) on fungal sexual reproduction and host infection remain largely unexplored. Here, we identified ROS metabolic genes and key signalling genes of MAPK and cAMP-PKA pathways in Curvularia lunata, which were NOX ClNOX1 and ClNOXR, superoxide dismutase ClSOD1 and catalase ClCAT4, redox-regulated transcription factor ClAP1, Ras small GTPases Clg2P, pheromone-response MAPK ClK1 and cAMP-PKA ClSCHA, and characterized the functions of these genes. The results showed that ClNOX1 localized to the plasma membrane. ClNOX1 and ClNOXR were involved in sexual reproduction and host infection via ClNOX1/ClNOXR-derived H(2) O(2) as well as MAPK and cAMP-PKA signalling pathways. H(2) O(2) acted as a signalling molecule to regulate sexual reproduction and host infection in C. lunata" |
| Keywords: | Catalase/metabolism Curvularia *Hydrogen Peroxide/metabolism NADPH Oxidases/metabolism Pheromones Reactive Oxygen Species/metabolism Reproduction/genetics Superoxide Dismutase/metabolism Transcription Factors/metabolism *ras Proteins/metabolism; |
| Notes: | "MedlineWang, Fen Liu, Kexin Wang, Jiahui Sun, Yuxin Xiao, Shuqin Xue, Chunsheng eng Research Support, Non-U.S. Gov't England 2022/06/09 Environ Microbiol. 2022 Sep; 24(9):4340-4355. doi: 10.1111/1462-2920.16089. Epub 2022 Jun 8" |
