« Previous AbstractAggregation pheromones have a non-linear effect on oviposition behavior in Drosophila melanogaster    Next AbstractA Lifetime of a Dispenser-Release Rates of Olive Fruit Fly-Associated Yeast Volatile Compounds and Their Influence on Olive Fruit Fly (Bactrocera oleae Rossi) Attraction »

EMBO J

Title:		"A novel regulator of G protein signalling in yeast, Rgs2, downregulates glucose-activation of the cAMP pathway through direct inhibition of Gpa2"
Author(s):		Versele M; de Winde JH; Thevelein JM;
Address:		"Laboratorium voor Moleculaire Celbiologie, Instituut voor Plantkunde en Microbiologie, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B-3001 Leuven-Heverlee, Flanders, Belgium"
Journal Title:		EMBO J
Year:		1999
Volume:		18
Issue:		20
Page Number:		5577 - 5591
DOI:		10.1093/emboj/18.20.5577
ISSN/ISBN:		0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking)
Abstract:		"We have characterized a novel member of the recently identified family of regulators of heterotrimeric G protein signalling (RGS) in the yeast Saccharomyces cerevisiae. The YOR107w/RGS2 gene was isolated as a multi-copy suppressor of glucose-induced loss of heat resistance in stationary phase cells. The N-terminal half of the Rgs2 protein consists of a typical RGS domain. Deletion and overexpression of Rgs2, respectively, enhances and reduces glucose-induced accumulation of cAMP. Overexpression of RGS2 generates phenotypes consistent with low activity of cAMP-dependent protein kinase A (PKA), such as enhanced accumulation of trehalose and glycogen, enhanced heat resistance and elevated expression of STRE-controlled genes. Deletion of RGS2 causes opposite phenotypes. We demonstrate that Rgs2 functions as a negative regulator of glucose-induced cAMP signalling through direct GTPase activation of the Gs-alpha protein Gpa2. Rgs2 and Gpa2 constitute the second cognate RGS-G-alpha protein pair identified in yeast, in addition to the mating pheromone pathway regulators Sst2 and Gpa1. Moreover, Rgs2 and Sst2 exert specific, non-overlapping functions, and deletion mutants in Rgs2 and Sst2 are complemented to some extent by different mammalian RGS proteins"
Keywords:		"Amino Acid Sequence Animals Cyclic AMP/metabolism Down-Regulation Fungal Proteins/*antagonists & inhibitors/genetics/*metabolism *GTP-Binding Protein alpha Subunits GTP-Binding Protein alpha Subunits, Gq-G11 GTP-Binding Proteins/*metabolism *GTPase-Activa;"
Notes:		"MedlineVersele, M de Winde, J H Thevelein, J M eng Research Support, Non-U.S. Gov't England 1999/10/16 EMBO J. 1999 Oct 15; 18(20):5577-91. doi: 10.1093/emboj/18.20.5577"