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Plant Physiol

Title:		12-Oxo-Phytodienoic Acid Acts as a Regulator of Maize Defense against Corn Leaf Aphid
Author(s):		Varsani S; Grover S; Zhou S; Koch KG; Huang PC; Kolomiets MV; Williams WP; Heng-Moss T; Sarath G; Luthe DS; Jander G; Louis J;
Address:		"Department of Entomology, University of Nebraska, Lincoln, Nebraska 68583. Boyce Thompson Institute, Cornell University, Ithaca, New York 14853. School of Integrated Plant Sciences, Cornell University, Ithaca, New York 14853. Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843. United States Department of Agriculture-Agricultural Research Service, Corn Host Plant Resistance Research Unit, Mississippi State, Mississippi 39762. Wheat, Sorghum, and Forage Research Unit, United States Department of Agriculture-Agricultural Research Service, Lincoln, Nebraska 68583. Department of Plant Science, Pennsylvania State University, University Park, Pennsylvania 16802. Department of Entomology, University of Nebraska, Lincoln, Nebraska 68583 joelouis@unl.edu. Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68583"
Journal Title:		Plant Physiol
Year:		2019
Volume:		20190114
Issue:		4
Page Number:		1402 - 1415
DOI:		10.1104/pp.18.01472
ISSN/ISBN:		1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking)
Abstract:		"The corn leaf aphid (CLA; Rhopalosiphum maidis) is a phloem sap-sucking insect that attacks many cereal crops, including maize (Zea mays). We previously showed that the maize inbred line Mp708, which was developed by classical plant breeding, provides enhanced resistance to CLA. Here, using electrophysiological monitoring of aphid feeding behavior, we demonstrate that Mp708 provides phloem-mediated resistance to CLA. Furthermore, feeding by CLA on Mp708 plants enhanced callose deposition, a potential defense mechanism utilized by plants to limit aphid feeding and subsequent colonization. In maize, benzoxazinoids (BX) or BX-derived metabolites contribute to enhanced callose deposition by providing heightened resistance to CLA. However, BX and BX-derived metabolites were not significantly altered in CLA-infested Mp708 plants, indicating BX-independent defense against CLA. Evidence presented here suggests that the constitutively higher levels of 12-oxo-phytodienoic acid (OPDA) in Mp708 plants contributed to enhanced callose accumulation and heightened CLA resistance. OPDA enhanced the expression of ethylene biosynthesis and receptor genes, and the synergistic interactions of OPDA and CLA feeding significantly induced the expression of the transcripts encoding Maize insect resistance1-Cysteine Protease, a key defensive protein against insect pests, in Mp708 plants. Furthermore, exogenous application of OPDA on maize jasmonic acid-deficient plants caused enhanced callose accumulation and heightened resistance to CLA, suggesting that the OPDA-mediated resistance to CLA is independent of the jasmonic acid pathway. We further demonstrate that the signaling function of OPDA, rather than a direct toxic effect, contributes to enhanced CLA resistance in Mp708"
Keywords:		"Acetates Animals Aphids/*physiology Benzoxazines/metabolism Cyclopentanes Ethylenes/biosynthesis Fatty Acids, Unsaturated/*physiology Fertility Glucans/*metabolism Herbivory Oxylipins Phloem/physiology Zea mays/*physiology;"
Notes:		"MedlineVarsani, Suresh Grover, Sajjan Zhou, Shaoqun Koch, Kyle G Huang, Pei-Cheng Kolomiets, Michael V Williams, W Paul Heng-Moss, Tiffany Sarath, Gautam Luthe, Dawn S Jander, Georg Louis, Joe eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2019/01/16 Plant Physiol. 2019 Apr; 179(4):1402-1415. doi: 10.1104/pp.18.01472. Epub 2019 Jan 14"