| Title: | Crystallization and preliminary X-ray diffraction studies of the transcriptional regulator TraR bound to its cofactor and to a specific DNA sequence |
| Author(s): | Vannini A; Volpari C; Gargioli C; Muraglia E; De Francesco R; Neddermann P; Di Marco S; |
| Address: | "Department of Biochemistry, IRBM P. Angeletti, 00040 Pomezia, Rome, Italy" |
| Journal Title: | Acta Crystallogr D Biol Crystallogr |
| DOI: | 10.1107/s0907444902010028 |
| ISSN/ISBN: | 0907-4449 (Print) 0907-4449 (Linking) |
| Abstract: | "TraR is an Agrobacterium tumefaciens transcriptional regulator which binds the pheromone N-3-oxooctanoyl-L-homoserine lactone (AAI) in response to the bacterial population density. The TraR-AAI complex dimerizes and interacts with a specific 18-base-pair DNA sequence (TraBox), activating promoters containing this site. TraR was overexpressed and purified from Escherichia coli. Crystals of the ternary complex, in which dimeric TraR-AAI is bound to the TraBox sequence, have been obtained by the vapour-diffusion method. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 66.99, b = 94.67, c = 209.66 A, with two (TraR-AAI)(2)-TraBox complexes in the asymmetric unit. A three-wavelength MAD data set for the seleno-L-methionine-substituted form has been collected to a resolution of 3 A. 20 of the 24 crystallographically independent selenium sites were located as part of the MAD-phasing process" |
| Keywords: | "Agrobacterium tumefaciens/chemistry Bacterial Proteins/*chemistry/isolation & purification Base Sequence Binding Sites Crystallization Crystallography, X-Ray DNA/chemistry Homoserine/*analogs & derivatives/chemistry Macromolecular Substances Recombinant P;" |
| Notes: | "MedlineVannini, Alessandro Volpari, Cinzia Gargioli, Cesare Muraglia, Ester De Francesco, Raffaele Neddermann, Petra Di Marco, Stefania eng 2002/07/24 Acta Crystallogr D Biol Crystallogr. 2002 Aug; 58(Pt 8):1362-4. doi: 10.1107/s0907444902010028. Epub 2002 Jul 20" |
