| Title: | Fate of selenate and selenite metabolized by Rhodobacter sphaeroides |
| Author(s): | Van Fleet-Stalder V; Chasteen TG; Pickering IJ; George GN; Prince RC; |
| Address: | "Chemistry Department and Texas Research Institute for Environmental Studies, Sam Houston State University, Huntsville, Texas 77341, USA" |
| DOI: | 10.1128/AEM.66.11.4849-4853.2000 |
| ISSN/ISBN: | 0099-2240 (Print) 1098-5336 (Electronic) 0099-2240 (Linking) |
| Abstract: | "Cultures of a purple nonsulfur bacterium, Rhodobacter sphaeroides, amended with approximately 1 or approximately 100 ppm selenate or selenite, were grown phototrophically to stationary phase. Analyses of culture headspace, separated cells, and filtered culture supernatant were carried out using gas chromatography, X-ray absorption spectroscopy, and inductively coupled plasma spectroscopy-mass spectrometry, respectively. While selenium-amended cultures showed much higher amounts of SeO(3)(2-) bioconversion than did analogous selenate experiments (94% uptake for SeO(3)(2-) as compared to 9.6% for SeO(4)(2-)-amended cultures from 100-ppm solutions), the chemical forms of selenium in the microbial cells were not very different except at exposure to high concentrations of selenite. Volatilization accounted for only a very small portion of the accumulated selenium; most was present in organic forms and the red elemental form" |
| Keywords: | "Chromatography, Gas/methods Culture Media/chemistry Rhodobacter sphaeroides/growth & development/*metabolism Selenic Acid Selenium Compounds/*metabolism Sodium Selenite/*metabolism Spectrum Analysis/methods;" |
| Notes: | "MedlineVan Fleet-Stalder, V Chasteen, T G Pickering, I J George, G N Prince, R C eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 2000/10/31 Appl Environ Microbiol. 2000 Nov; 66(11):4849-53. doi: 10.1128/AEM.66.11.4849-4853.2000" |
