| Title: | Exhaled breath profiling in diagnosing wheezy preschool children |
| Author(s): | van de Kant KD; van Berkel JJ; Jobsis Q; Lima Passos V; Klaassen EM; van der Sande L; van Schayck OC; de Jongste JC; van Schooten FJ; Derks E; Dompeling E; Dallinga JW; |
| Address: | "Dept of Paediatric Pulmonology, School for Public Health and Primary Care, CAPHRI, Maastricht University Medical Centre, Maastricht, The Netherlands" |
| DOI: | 10.1183/09031936.00122411 |
| ISSN/ISBN: | 1399-3003 (Electronic) 0903-1936 (Linking) |
| Abstract: | "Although wheeze is common in preschool children, the underlying pathophysiology has not yet been disentangled. Volatile organic compounds (VOCs) in exhaled breath may serve as noninvasive markers of early wheeze. We aimed to assess the feasibility of VOC collection in preschool children, and to study whether a VOC profile can differentiate between children with and without recurrent wheeze. We included children (mean (range) age 3.3 (1.9-4.5) yrs) with (n=202) and without (n=50) recurrent wheeze. Exhaled VOCs were analysed by gas chromatography-time-of-flight mass spectrometry. VOC profiles were generated by ANOVA simultaneous component analysis (ASCA) and sparse logistic regression (SLR). Exhaled breath collection was possible in 98% of the children. In total, 913 different VOCs were detected. The signal-to-noise ratio improved after correction for age, sex and season using ASCA pre-processing. An SLR model with 28 VOCs correctly classified 83% of the children (84% sensitivity, 80% specificity). After six-fold cross-validation, 73% were correctly classified (79% sensitivity, 50% specificity). Assessment of VOCs in exhaled breath is feasible in young children. VOC profiles are able to distinguish children with and without recurrent wheeze with a reasonable accuracy. This proof of principle paves the way for additional research on VOCs in preschool wheezing" |
| Keywords: | "Breath Tests Child, Preschool Exhalation Feasibility Studies Female Humans Infant Male Prospective Studies Respiratory Sounds/*diagnosis Volatile Organic Compounds/*analysis;" |
| Notes: | "Medlinevan de Kant, Kim D G van Berkel, Joep J B N Jobsis, Quirijn Lima Passos, Valeria Klaassen, Ester M M van der Sande, Linda van Schayck, Onno C P de Jongste, Johan C van Schooten, Frederik Jan Derks, Eduard Dompeling, Edward Dallinga, Jan W eng Clinical Trial Research Support, Non-U.S. Gov't England 2013/01/02 Eur Respir J. 2013 Jan; 41(1):183-8. doi: 10.1183/09031936.00122411" |
