| Title: | "Borrelidin inhibits a cyclin-dependent kinase (CDK), Cdc28/Cln2, of Saccharomyces cerevisiae" |
| Author(s): | Tsuchiya E; Yukawa M; Miyakawa T; Kimura KI; Takahashi H; |
| Address: | "Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Japan. stsuchi@hiroshima-u.ac.jp" |
| DOI: | 10.7164/antibiotics.54.84 |
| ISSN/ISBN: | 0021-8820 (Print) 0021-8820 (Linking) |
| Abstract: | "We identified borrrelidin, a member of macrolide antibiotic, as an inhibitor of a cyclin-dependent kinase of the budding yeast, Cdc28/Cln2. A 50% inhibition concentration (IC50) of borrelidin for Cdc28/Cln2 was 24 microM. In addition, borrelidin arrests both haploid and diploid cells in G1 phase at the point indistinguishable from that of alpha-mating pheromone, at concentrations not affecting the gross protein synthesis. Although the inhibition of CDK activity may not be a solo cause of the G1 arrest, our results indicate that borrelidin is a potential lead compound for developing novel CDK inhibitors of higher eukaryotes" |
| Keywords: | "CDC28 Protein Kinase, S cerevisiae/*antagonists & inhibitors Enzyme Inhibitors/*pharmacology Fatty Alcohols/*pharmacology Fungal Proteins/biosynthesis/drug effects G1 Phase/drug effects Inhibitory Concentration 50 Mating Factor Peptides/drug effects/metab;" |
| Notes: | "MedlineTsuchiya, E Yukawa, M Miyakawa, T Kimura, K I Takahashi, H eng Research Support, Non-U.S. Gov't England 2001/03/28 J Antibiot (Tokyo). 2001 Jan; 54(1):84-90. doi: 10.7164/antibiotics.54.84" |
