| Title: | Interference in pheromone-responsive conjugation of a high-level bacitracin resistant Enterococcus faecalis plasmid of poultry origin |
| Author(s): | Tremblay CL; Archambault M; |
| Address: | "GREMIP research group, Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte Street, Saint-Hyacinthe, QC J2S 7C6, Canada. cl.tremblay@umontreal.ca" |
| Journal Title: | Int J Environ Res Public Health |
| ISSN/ISBN: | 1660-4601 (Electronic) 1661-7827 (Print) 1660-4601 (Linking) |
| Abstract: | "The current study reports on contact interference of a high-level bacitracin- resistant pheromone-responsive plasmid of Enterococcus faecalis strain 543 of poultry origin during conjugative transfer of bcr antimicrobial resistance genes using a polyclonal antiserum aggregation substance(44-560) (AS). After induction with pheromones produced by the recipient strain E. faecalis JH2-2, clumping of the donor E. faecalis strain 543 was observed as well as high transfer frequencies of bcr in short time broth mating. Filter mating assays from donor strain E. faecalis 543 to the recipient strain E. faecalis JH2-2 revealed conjugative transfer of asa1 (AS), bcrRAB and traB (negative regulator pheromone response) genes. The presence of these genes in transconjugants was confirmed by antimicrobial susceptibility testing, PCR, Southern hybridization and sequencing. A significant reduction in formation of aggregates was observed when the polyclonal anti-AS(44-560) was added in the pheromone-responsive conjugation experiments as compared to the induced state. Moreover, interference of anti-AS(44-560) antibodies in pheromone-responsive conjugation was demonstrated by a reduction in horizontal transfer of asa1 and bcr genes between E. faecalis strain 543 and E. faecalis JH2-2. Reducing the pheromone-responsive conjugation of E. faecalis is of interest because of its clinical importance in the horizontal transfer of antimicrobial resistance" |
| Keywords: | "Animals Anti-Bacterial Agents/pharmacology Bacitracin/pharmacology Chickens/*microbiology Drug Resistance, Bacterial/*genetics Enterococcus faecalis/*genetics Genes, Bacterial Microbial Sensitivity Tests Pheromones/*pharmacology Plasmids;" |
| Notes: | "MedlineTremblay, Cindy-Love Archambault, Marie eng Research Support, Non-U.S. Gov't Switzerland 2013/09/14 Int J Environ Res Public Health. 2013 Sep 11; 10(9):4245-60. doi: 10.3390/ijerph10094245" |
