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J Chromatogr A

Title:		Enhanced resolution comprehensive two-dimensional gas chromatography applied to the analysis of roasted coffee volatiles
Author(s):		Tranchida PQ; Purcaro G; Conte L; Dugo P; Dugo G; Mondello L;
Address:		"Dipartimento Farmaco-chimico, Facolta di Farmacia, Universita di Messina, viale Annunziata, 98168 Messina, Italy"
Journal Title:		J Chromatogr A
Year:		2009
Volume:		20090623
Issue:		43
Page Number:		7301 - 7306
DOI:		10.1016/j.chroma.2009.06.056
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"The present research is based on the full exploitation of the separation power of a 0.05 mm internal diameter (ID) capillary, as a comprehensive two-dimensional (2D) GC (GC x GC) secondary column, with the objective of attaining very high-resolution second dimension separations. The aim was achieved by using a split-flow system developed in previous research [P.Q. Tranchida, A. Casilli, P. Dugo, G. Dugo, L. Mondello, Anal. Chem. 79 (2007) 2266], and a dual-oven GC x GC instrument. The column combination employed consisted of a polar 30 m x 0.25 mm ID column connected, by means of a T union, to a detector-linked high-resolution 1.1 m x 0.05 mm ID apolar analytical column and to a 0.33 m x 0.05 mm ID retention gap; the latter was connected to a manually operated split valve. As previously demonstrated, the use of a split valve enables the regulation of gas flows through both analytical columns, generating the most appropriate gas linear velocities. Comprehensive 2D GC experiments were carried out on Arabica roasted coffee volatiles (previously extracted by means of solid-phase microextraction) with the split-valve closed (equal to what can be defined as conventional GC x GC) and with the split-valve opened at various degrees. The reasons why it is absolutely not effective to use a 0.05 mm ID column as second dimension in a conventional GC x GC instrument will be discussed and demonstrated. On the contrary, the use of a 0.05 mm ID column as second dimension, under ideal conditions in a split-flow, twin-oven system, will also be illustrated and discussed"
Keywords:		"Chromatography, Gas/*methods Coffea/*chemistry Coffee/*chemistry Plant Extracts/*analysis Seeds/*chemistry Solid Phase Microextraction Volatile Organic Compounds/*analysis;"
Notes:		"MedlineTranchida, Peter Quinto Purcaro, Giorgia Conte, Lanfranco Dugo, Paola Dugo, Giovanni Mondello, Luigi eng Research Support, Non-U.S. Gov't Netherlands 2009/07/15 J Chromatogr A. 2009 Oct 23; 1216(43):7301-6. doi: 10.1016/j.chroma.2009.06.056. Epub 2009 Jun 23"