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Genes Genet Syst

Title:		"Conserved cis-regulatory elements of two odorant-binding protein genes, Obp57d and Obp57e, in Drosophila"
Author(s):		Tomioka S; Aigaki T; Matsuo T;
Address:		"Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami Osawa,Hachioji, Tokyo 192-0364, Japan"
Journal Title:		Genes Genet Syst
Year:		2012
Volume:		87
Issue:		5
Page Number:		323 - 329
DOI:		10.1266/ggs.87.323
ISSN/ISBN:		1880-5779 (Electronic) 1341-7568 (Linking)
Abstract:		"Insect odorant-binding proteins function in the sensing of odors, tastes, and pheromones. Genes encoding two odorant-binding proteins, Obp57d and Obp57e, were identified to be involved in the behavioral adaptation of Drosophila sechellia to its host plant. The two genes are expressed in cells associated with taste sensilla on the legs, and the expression pattern in the legs is conserved among closely related species. To identify the cis-regulatory elements necessary for the expression in the leg sensilla, the promoter sequences of Obp57d and Obp57e were compared among species. Two types of conserved sequence-motifs were found as candidate cis-regulatory elements. Functions of these conserved elements in the promoters of D. melanogaster Obp57d and Obp57e were examined by using a newly constructed vector that combines the advantages of phiC31 integrase-based transformation and gypsy transposable-element-derived insulators. By GFP-reporter assay using the new vector, it was confirmed that these conserved elements are necessary for the expression in the legs, working synergistically with each other to affect the expression level. Single-nucleotide substitutions in these elements dramatically changed the promoter activity. These results provide insight into the molecular mechanism for evolution of adaptive behavior via modulation of OBP expression levels"
Keywords:		"Animals Animals, Genetically Modified Base Sequence *Conserved Sequence Drosophila/*genetics Drosophila Proteins/*genetics Female Gene Expression Gene Expression Regulation Gene Order Genes, Reporter Genetic Vectors Male Nucleotide Motifs Promoter Regions;"
Notes:		"MedlineTomioka, Sachiko Aigaki, Toshiro Matsuo, Takashi eng Research Support, Non-U.S. Gov't Japan 2013/02/16 Genes Genet Syst. 2012; 87(5):323-9. doi: 10.1266/ggs.87.323"