| Title: | "Floral micromorphology and transcriptome analyses of a fragrant Vandaceous Orchid, Vanda Mimi Palmer, for its fragrance production sites" |
| Author(s): | Toh C; Mohd-Hairul AR; Ain NM; Namasivayam P; Go R; Abdullah NAP; Abdullah MO; Abdullah JO; |
| Address: | "Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia. Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Gambang, 26300, Kuantan, Pahang, Malaysia. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, 97008, Bintulu, Sarawak, Malaysia. Malaysian Palm Oil Board, 6 Persiaran Institusi, Bandar Baru Bangi, 43000, Kajang, Selangor Darul Ehsan, Malaysia. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia. janna@upm.edu.my" |
| DOI: | 10.1186/s13104-017-2872-6 |
| ISSN/ISBN: | 1756-0500 (Electronic) 1756-0500 (Linking) |
| Abstract: | "BACKGROUND: Vanda Mimi Palmer (VMP) is commercially valuable for its strong fragrance but little is known regarding the fragrance production and emission sites on the flowers. RESULTS: Olfactory perception detected fragrance only from the petals and sepals. Light and Environmental Scanning Electron microscopy analyses on fresh tissues showed distributions of stomata and trichomes concentrated mostly around the edges. These results paralleled the rich starch deposits and intense neutral red stain, indicating strong fragrance and trichomes as potential main fragrance release sites. Next Generation Sequencing (NGS) transcriptomic data of adaxial and abaxial layers of the tissues showed monoterpene synthase transcripts specifically linalool and ocimene synthases distributed throughout the tissues. qPCR analyses taken at different time points revealed high levels of linalool and ocimene synthases transcripts in the early morning with maximal level at 4.00 am but remained low throughout daylight hours. CONCLUSIONS: Knowledge of the VMP floral anatomy and its fragrance production characteristics, which complemented our previous molecular and biochemical data on VMP, provided additional knowledge on how fragrance and flower morphology are closely intertwined. Further investigation on the mechanisms of fragrance biosynthesis and interaction of potential pollinators would elucidate the evolution of the flower morphology to maximize the reproduction success of this plant" |
| Keywords: | *Flowers/genetics/physiology/ultrastructure Gene Expression Profiling/*methods *Odorants *Orchidaceae/genetics/physiology/ultrastructure Fragrance Monoterpene synthase Morphology Orchid Stomata Trichome; |
| Notes: | "MedlineToh, Conie Mohd-Hairul, Ab Rahim Ain, Nooraini Mohd Namasivayam, Parameswari Go, Rusea Abdullah, Nur Ashikin Psyquay Abdullah, Meilina Ong Abdullah, Janna Ong eng England 2017/11/04 BMC Res Notes. 2017 Nov 2; 10(1):554. doi: 10.1186/s13104-017-2872-6" |
