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J Food Sci

Title:		Detection of Brettanomyces spp. in red wines using real-time PCR
Author(s):		Tofalo R; Schirone M; Corsetti A; Suzzi G;
Address:		"Dept of Scienze degli Alimenti, Univ. degli Studi di Teramo, Via CR Lerici 1, 64023 Mosciano Sant'Angelo, Teramo, Italy. rtofalo@unite.it"
Journal Title:		J Food Sci
Year:		2012
Volume:		20120821
Issue:		9
Page Number:		M545 - M549
DOI:		10.1111/j.1750-3841.2012.02871.x
ISSN/ISBN:		1750-3841 (Electronic) 0022-1147 (Linking)
Abstract:		"The question if the 'Brett character' is a favorable wine attribute is one of the most controversial issues and it is currently addressed by many researches. Actually, the presence of Brettanomyces/Dekkera in wine during barrel aging is often associated to detrimental organoleptic characteristics depending on the release of volatile phenols (for example, 4-ethylphenol and 4-ethylguaiacol); for that reason the possibility to rapidly detect the yeast at the early stage of wine production could allow preventive actions to reduce wine spoilage. In this work, 25 and 5 samples from conventional and organic vineyards, respectively, all suspected to be spoiled by Brettanomyces/Dekkera spp., were analyzed using both culture-dependent and culture-independent techniques. In particular, a DNA extraction protocol and a real-time quantitative PCR (qPCR) assay to directly detect and quantify B. bruxellensis were optimized. Results showed that B. bruxellensis was present in 22 of 30 samples, ranging from 10 to 10(4) CFU/mL, lower values being found in organic wines (10 to 10(2) CFU/mL). Overall, qPCR was proved to be a useful and valuable wine control system, since 12 samples were recorded as positive for yeast presence when analyzed by qPCR and negative in case of plate count analyses. PRACTICAL APPLICATION: Brettanomyces cells were detected using a qPCR method, optimized in this study, which allows to obtain results quickly"
Keywords:		"Brettanomyces/*isolation & purification DNA, Fungal/isolation & purification Dekkera/*isolation & purification Food Contamination/analysis Food Microbiology Guaiacol/analogs & derivatives/analysis Phenols/analysis Real-Time Polymerase Chain Reaction/*meth;"
Notes:		"MedlineTofalo, Rosanna Schirone, Maria Corsetti, Aldo Suzzi, Giovanna eng Research Support, Non-U.S. Gov't 2012/08/23 J Food Sci. 2012 Sep; 77(9):M545-9. doi: 10.1111/j.1750-3841.2012.02871.x. Epub 2012 Aug 21"