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Insect Biochem Mol Biol

Title:		"Isolation and endocrine regulation of an HMG-CoA synthase cDNA from the male Jeffrey pine beetle, Dendroctonus jeffreyi (Coleoptera: Scolytidae)"
Author(s):		Tittiger C; O'Keeffe C; Bengoa CS; Barkawi LS; Seybold SJ; Blomquist GJ;
Address:		"Department of Biochemistry, University of Nevada, Reno, NV 89557-0014, USA. crt@unr.edu"
Journal Title:		Insect Biochem Mol Biol
Year:		2000
Volume:		30
Issue:		12
Page Number:		1203 - 1211
DOI:		10.1016/s0965-1748(00)00099-0
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"We have isolated a full length 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) cDNA from the male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins, and studied the effects of topical applications of juvenile hormone III (JH III) on its expression. The predicted translation product of this apparently single copy gene has 63% and 58% identity with HMG-S1 and HMG-S2 from Blattella germanica (L.), and 61% identity with Drosophila melanogaster Hmgs. HMG-S transcript levels remain uniformly low in JH III-treated and control D. jeffreyi females, but are induced approximately 2.5- to 5-fold in JH III-treated males. JH III causes a dose- and time-dependent increase in HMG-S transcripts in the male metathoracic-abdominal region. Since monoterpenoid pheromone precursor synthesis and HMG-CoA reductase expression are under the control of JH III in the metathorax of Ips bark beetles, the observed HMG-S expression pattern suggests that the isoprenoid pathway is similarly important for semiochemical production in D. jeffreyi"
Keywords:		"Amino Acid Sequence Animals Base Sequence Coleoptera/*enzymology/genetics DNA, Complementary Endocrine System/metabolism Gene Dosage *Gene Expression Regulation, Enzymologic Humans Hydroxymethylglutaryl-CoA Synthase/*genetics/isolation & purification/meta;"
Notes:		"MedlineTittiger, C O'Keeffe, C Bengoa, C S Barkawi, L S Seybold, S J Blomquist, G J eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2000/10/25 Insect Biochem Mol Biol. 2000 Dec; 30(12):1203-11. doi: 10.1016/s0965-1748(00)00099-0"