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Insects

Title:		OBP14 (Odorant-Binding Protein) Sensing in Adelphocoris lineolatus Based on Peptide Nucleic Acid and Graphene Oxide
Author(s):		Tian W; Zhang T; Gu S; Guo Y; Gao X; Zhang Y;
Address:		"Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"
Journal Title:		Insects
Year:		2021
Volume:		20210508
Issue:		5
Page Number:		-
DOI:		10.3390/insects12050422
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JH?a=. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest"
Keywords:		Adelphocoris lineolatus AlinOBP14 Plus-C OBPs graphene oxide (GO) in vitro in vivo juvenile hormone mRNA biosensor peptide nucleic acid (PNA) terpenoids;
Notes:		"PubMed-not-MEDLINETian, Wenhua Zhang, Tao Gu, Shaohua Guo, Yuyuan Gao, Xiwu Zhang, Yongjun eng 31772176, 31972338/National Natural Science Foundation of China/ 2019YFD0300100/National Key Research and Development Program of China/ Switzerland 2021/06/03 Insects. 2021 May 8; 12(5):422. doi: 10.3390/insects12050422"