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Methods Mol Biol


Title:Culture systems: air quality
Author(s):Thomas T;
Address:"Reproductive Technology Laboratory, Martha Jefferson Hospital, Charlottesville, VA, USA. Ted.thomas@mjh.org"
Journal Title:Methods Mol Biol
Year:2012
Volume:912
Issue:
Page Number:313 - 324
DOI: 10.1007/978-1-61779-971-6_17
ISSN/ISBN:1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:"Poor laboratory air quality is a known hazard to the culture of human gametes and embryos. Embryologists and chemists have employed analytical methods for identifying and measuring bulk and select air pollutants to assess the risk they pose to the embryo culture system. However, contaminant concentrations that result in gamete or embryotoxicity are poorly defined. Combating the ill effects of poor air quality requires an understanding of how toxicants can infiltrate the laboratory, the incubator, and ultimately the culture media. A further understanding of site-specific air quality can then lead to the consideration of laboratory design and management strategies that can minimize the deleterious effects that air contamination may have on early embryonic development in vitro"
Keywords:"Air Pollution, Indoor/*analysis Culture Media/chemistry Embryo Culture Techniques/instrumentation/*methods Environment, Controlled Fertilization in Vitro Humans Incubators Laboratories Particulate Matter/analysis Pressure Ultraviolet Rays Volatile Organic;"
Notes:"MedlineThomas, Theodore eng 2012/07/26 Methods Mol Biol. 2012; 912:313-24. doi: 10.1007/978-1-61779-971-6_17"

 
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