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Int Microbiol

Title:		Involvement of lytic enzymes and secondary metabolites produced by Trichoderma spp. in the biological control of Pythium myriotylum
Author(s):		Tchameni SN; Cotarlet M; Ghinea IO; Bedine MAB; Sameza ML; Borda D; Bahrim G; Dinica RM;
Address:		"Laboratory of Biochemistry, Faculty of Science, University of Douala Cameroon, PO Box 24157, Douala, Cameroon. Faculty of Science and Environment 'Dunarea de Jos', University of Galati, Romania, Domneasca Street, 47, 800008, Galati, Romania. Faculty of Food Science and Engineering, 'Dunarea de Jos', University of Galati, Romania. Domneasca Street, 47, 800008, Galati, Romania. Laboratory of Phytobiochemistry, Faculty of Science, University of Yaounde I, PO Box 812, Yaounde, Cameroon. Faculty of Science and Environment 'Dunarea de Jos', University of Galati, Romania, Domneasca Street, 47, 800008, Galati, Romania. Rodica.Dinica@ugal.ro"
Journal Title:		Int Microbiol
Year:		2020
Volume:		20190702
Issue:		2
Page Number:		179 - 188
DOI:		10.1007/s10123-019-00089-x
ISSN/ISBN:		1618-1905 (Electronic) 1139-6709 (Linking)
Abstract:		"This investigation was aimed to evaluate the antimicrobial activities and involvement of extracellular lytic enzymes produced by four strains of Trichoderma in the inhibition of Pythium myriotylum. Antagonistic effects were tested by dual culture. Activities of lytic enzymes were evaluated from the filtrate of each strain after cultivation in selected media. Organic extracts were obtained from liquid media subsequent to the cultivation of Trichoderma in potato dextrose broth (PDB). Non-volatile organic compounds such as polyphenols and flavonoids were evaluated spectrophotometrically while volatile organic compounds (VOCs) were analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The antimicrobial activity of the organic extracts was determined using the poisoning method. Results have shown that all the strains were antagonists against P. myriotylum. T. erinaceum (IT-58), T. gamsii (IT-62), T. afroharzianum (P8), and T. harzianum (P11) that were found to produce cellulase, protease, and xylanase. Over 20 compounds were identified in each extract, including esters, lactones, and organic acids. The organic extracts also contained high amounts of polyphenolic compounds and flavonoids and significantly inhibited the mycelial growth of P. myriotylum. The minimal inhibition concentrations were 80 mug/muL, 40 mug/muL, 20 mug/muL, and 10 mug/muL, for extracts obtained from T. erinaceum (IT-58), T. gamsii (IT-62), T. afroharzianum (P8), and T. harzianum (P11), respectively. There was significant correlation between the production of total polyphenol and flavonoid content and the antagonistic effects of the tested strains"
Keywords:		"Antifungal Agents/chemistry/pharmacology Biological Control Agents Cellulase/metabolism Complex Mixtures/chemistry/pharmacology Endo-1, 4-beta Xylanases/metabolism Enzymes/metabolism Microbial Sensitivity Tests Peptide Hydrolases/metabolism Plant Diseases/;"
Notes:		"MedlineTchameni, Severin Nguemezi Cotarlet, Mihaela Ghinea, Ioana Otilia Bedine, Marie Ampere Boat Sameza, Modeste Lambert Borda, Daniela Bahrim, Gabriela Dinica, Rodica Mihaela eng Switzerland 2019/07/04 Int Microbiol. 2020 May; 23(2):179-188. doi: 10.1007/s10123-019-00089-x. Epub 2019 Jul 2"