« Previous Abstract"The evolution of pollution profile and health risk assessment for three groups SVOCs pollutants along with Beijiang River, China"    Next Abstract"Effects of Early Pollution Control Measures on Secondary Species of PM(2.5) in Jiaozuo, China" »

R Soc Open Sci

Title:		Assessing urinary odours across the oestrous cycle in a mouse model using portable and benchtop gas chromatography-mass spectrometry
Author(s):		Tang J; Poirier AC; Duytschaever G; Moreira LAA; Nevo O; Melin AD;
Address:		"Department of Anthropology and Archaeology, University of Calgary, Canada. German Centre of Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Germany. Institute of Biodiversity, Friedrich Schiller University Jena, Germany. Department of Medical Genetics, University of Calgary, Canada. Alberta Children's Hospital Research Institute, University of Calgary, Canada"
Journal Title:		R Soc Open Sci
Year:		2021
Volume:		20210901
Issue:		9
Page Number:		210172 -
DOI:		10.1098/rsos.210172
ISSN/ISBN:		2054-5703 (Print) 2054-5703 (Electronic) 2054-5703 (Linking)
Abstract:		"For female mammals, communicating the timing of ovulation is essential for reproduction. Olfactory communication via volatile organic compounds (VOCs) can play a key role. We investigated urinary VOCs across the oestrous cycle using laboratory mice. We assessed the oestrous stage through daily vaginal cytology and analysed urinary VOCs using headspace gas chromatography-mass spectrometry (GC-MS), testing a portable GC-MS against a benchtop system. We detected 65 VOCs from 40 samples stored in VOC traps and analysed on a benchtop GC-MS, and 15 VOCs from 90 samples extracted by solid-phase microextraction (SPME) and analysed on a portable GC-MS. Only three compounds were found in common between the two techniques. Urine collected from the fertile stages of the oestrous cycle had increased quantities of a few notable VOCs compared with urine from non-fertile stages. These VOCs may be indicators of fertility. However, we did not find significant differences in chemical composition among oestrous stages. It is possible that changes in VOC abundance were too small to be detected by our analytical methods. Overall, the use of VOC traps combined with benchtop GC-MS was the more successful of the two methods, yet portable GC-MS systems may still have utility for some in situ applications"
Keywords:		gas chromatography-mass spectrometry house mouse olfactory communication solid-phase microextraction volatile organic compounds;
Notes:		"PubMed-not-MEDLINETang, Jia Poirier, Alice C Duytschaever, Gwen Moreira, Lais A A Nevo, Omer Melin, Amanda D eng England 2021/09/21 R Soc Open Sci. 2021 Sep 1; 8(9):210172. doi: 10.1098/rsos.210172. eCollection 2021 Sep"