| Title: | A new headspace solid-phase microextraction coupled with gas chromatography-tandem mass spectrometry method for the simultaneous quantification of 21 microbial volatile organic compounds in urine and blood |
| Author(s): | Tabbal S; El Aroussi B; Bouchard M; Marchand G; Haddad S; |
| Address: | "Department of Environmental and Occupational Health (DSEST), University of Montreal, Montreal, Quebec, Canada; Centre de recherche en sante publique (CReSP), Montreal, Quebec, Canada. Institut de recherche Robert-Sauve en sante et en securite du travail (IRSST), Montreal, Quebec, Canada. Department of Environmental and Occupational Health (DSEST), University of Montreal, Montreal, Quebec, Canada; Centre de recherche en sante publique (CReSP), Montreal, Quebec, Canada. Electronic address: sami.haddad@umontreal.ca" |
| DOI: | 10.1016/j.chemosphere.2022.133901 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "Mold growth can cause the development of several metabolites including microbial volatile organic compounds (mVOCs). These latter may be considered as potential biomarkers of fungal presence and have been detected in human biological matrices such as urine and blood. Exposure to molds and their metabolites (e.g., mVOCs, mycotoxins) in occupational settings, is responsible for several health effects. Thus, this exposure cannot be neglected and must be evaluated. Herein, a method has been developed to quantify 21 mVOCs in urine and human blood by headspace solid phase micro-extraction (HS-SPME) coupled with gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The parameters influencing the extraction process, such as the type of fiber, the incubation and extraction time and temperature and the desorption time, have been optimized to ensure better mVOCs extraction. The developed method showed good linearity over the concentration range of the compounds (R(2) > 0.995) for all the mVOCs in all the matrices. The low limits of detection (LOD) ranging from 0.7 to 417 ng/L in urine and from 1 to 507 ng/L in blood, make the developed methods sensitive and effective for biomonitoring of exposure at low levels. Recoveries, at low and high concentrations, were between 87% and 120% in urine and between 83% and 118% in blood. The repeatability and the intermediate precision in terms of coefficients of variation (CV%) was lower than 13% and 8.58% respectively for all compounds in all matrices. These values show satisfactory accuracy and precision of the developed method. Thus, this practical, simple, and sensitive method is well suited for the simultaneous quantification of target mVOCs" |
| Keywords: | Fungi Gas Chromatography-Mass Spectrometry/methods Humans Limit of Detection *Solid Phase Microextraction/methods Tandem Mass Spectrometry *Volatile Organic Compounds/analysis Blood Hs-spme-gc-ms/ms Microbial volatile organic compounds (mVOCs) Optimizatio; |
| Notes: | "MedlineTabbal, Sarah El Aroussi, Badr Bouchard, Michele Marchand, Genevieve Haddad, Sami eng England 2022/02/11 Chemosphere. 2022 Jun; 296:133901. doi: 10.1016/j.chemosphere.2022.133901. Epub 2022 Feb 7" |
