| Title: | Differential expression profiling of Oxycarenus laetus Kirby (Hemiptera: Lygaeidae) upon exposure to gossypol |
| Author(s): | Sureshan SC; Mohideen HS; Ramya M; |
| Address: | "Bioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India. Bioinformatics and Entomoinformatics Lab, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India. habeeb_skm@yahoo.co.in. Molecular Genetics Laboratory, Department of Genetic Engineering, College of Engineering and Technology, Faculty of Engineering and Technology, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chengalpattu, Chennai, TN, 603203, India" |
| DOI: | 10.1007/s11033-022-07322-3 |
| ISSN/ISBN: | 1573-4978 (Electronic) 0301-4851 (Linking) |
| Abstract: | "BACKGROUND: Gossypium hirsutum seeds are rich in gossypol. In addition to its diverse beneficial properties, it is a known anti-fertility inducing agent in humans. Oxycarenus laetus feeds on the cottonseeds and yet its courtship, mating and reproduction is unaffected. METHODS AND RESULTS: In this study, we performed a transcriptome profiling of O. laetus fed on Abutilon indicum (AB-no gossypol), G. hirsutum (GH-natural gossypol) and 1400 ppm commercial gossypol-soaked GH seeds (GHGO). Illumina NextSeq-500 paired-end 75 bp reads were generated and de novo assembled (48,214 genes) to identify the differentially expressed transcripts (DET) between the samples. Gene enrichment, KEGG pathway and cluster profiling of the DETs resulted in the identification of vital genes involved in the detoxification, pheromone biosynthesis, cuticle protein in the GHGO sample. Cyp4C1, Cyp6a13, Cyp6a14, Cyp4g15, Cyp4em8, Cyp303a1 were the detoxification related genes identified. Similarly, SDR dehydrogenase family 11 and fatty acid synthase in pheromone biosynthesis and cuticle proteins (RR1 and RR2) coding transcripts were found to be differentially expressed. CONCLUSION: This is the first study to report the expression of genes induced by gossypol in O. laetus. Based on the findings from the DET analysis, we conclude that the detoxification related genes of gossypol treated samples were affected" |
| Keywords: | Animals Humans Gene Expression Profiling Gossypium/genetics/metabolism *Gossypol/analysis/metabolism/pharmacology *Hemiptera/genetics/metabolism Pheromones/metabolism Bioinformatics Detoxification Differentially expressed transcripts Gossypol Oxycarenus l; |
| Notes: | "MedlineSureshan, Shruthi Chalil Mohideen, Habeeb Shaik Ramya, Mohandass eng Netherlands 2022/03/16 Mol Biol Rep. 2022 Jun; 49(6):4727-4735. doi: 10.1007/s11033-022-07322-3. Epub 2022 Mar 15" |
