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Insects

Title:		GOBP1 from the Variegated Cutworm Peridroma saucia (Hubner) (Lepidoptera: Noctuidae) Displays High Binding Affinities to the Behavioral Attractant (Z)-3-Hexenyl acetate
Author(s):		Sun YL; Dong JF; Song YQ; Wang SL;
Address:		"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, China. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China"
Journal Title:		Insects
Year:		2021
Volume:		20211015
Issue:		10
Page Number:		-
DOI:		10.3390/insects12100939
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"The variegated cutworm Peridroma saucia (Hubner) is a worldwide pest that causes serious damage to many crops. To recognize sex pheromones and host plant volatiles, insects depend on olfactory chemoreception involving general odorant-binding proteins (GOBPs). In this study, PsauGOBP1 was cloned from the adult antennae of P. saucia. RT-qPCR and Western-blot analysis showed that PsauGOBP1 was specifically and equally expressed in the adult antennae of both females and males. Fluorescence competitive-binding assays with sex pheromones and host plant volatiles demonstrated that PsauGOBP1 bound to six host plant volatiles: (Z)-3-hexenyl acetate (K(D) = 4.0 +/- 0.1 muM), citral (K(D) = 5.6 +/- 0.4 muM), farnesol (K(D) = 6.4 +/- 0.6 muM), nonanal (K(D) = 6.8 +/- 0.3 muM), (Z)-3-hexen-1-ol (K(D) = 8.5 +/- 0.6 muM), and benzaldehyde (K(D) = 9.4 +/- 0.5 muM). Electroantennogram recordings with the six host plant volatiles indicated that (Z)-3-hexenyl acetate elicited the strongest responses from both male and female antennae. Further bioassays using Y-tube olfactometers showed that (Z)-3-hexenyl acetate was attractive to adult P. saucia of both sexes. These results suggest that PsauGOBP1 might be involved in detecting host plant volatiles and that (Z)-3-hexenyl acetate might serve as a potential attractant for the biological control of P. saucia"
Keywords:		EAG recording Peridroma saucia Western blot behavioral response fluorescence competitive binding general odorant-binding protein real-time quantitative PCR;
Notes:		"PubMed-not-MEDLINESun, Ya-Lan Dong, Jun-Feng Song, Yue-Qin Wang, Shao-Li eng 32001917/National Natural Science Foundation of China/ CARS-25/China Agriculture Research System/ 202102110069/Scientific and Technological Research Projects in Henan Province/ Switzerland 2021/10/24 Insects. 2021 Oct 15; 12(10):939. doi: 10.3390/insects12100939"