| Title: | "Function of mevalonate pathway genes in the synthesis of frontalin in Chinese white pine beetle, Dendroctonus armandi (curculionidae: Scolytinae)" |
| Author(s): | Sun Y; Fu D; Kang X; Liu B; Ning H; Chen H; |
| Address: | "College of Forestry, Northwest A and F University, Yangling, Shaanxi, China. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, Guangdong, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "The Chinese white pine beetle (Dendroctonus armandi Tsai and Li) is a significant pest of pine forests in the Qinling and Bashan Mountains of China. Adult males commonly produce frontalin using precursors synthesized through the mevalonate pathway, which is regulated by juvenile hormone III (JHIII). In this study, the expression levels of mevalonate pathway genes were quantified after phloem feeding and topical application of the JHIII solution. The frontalin was quantified by gas chromatography-mass spectrometry. Both the phloem feeding and JHIII treatments produced an evident upregulation in the male gut, mainly in 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR). Moreover, HMGS, HMGR, isopentenyl diphosphate isomerase, and geranyl diphosphate synthase/farnesyl diphosphate synthase were upregulated in fed and JHIII-stimulated males of D. armandi under both conditions (solitary and paired). The expression levels were higher in paired compared to solitary males. Males had higher expression levels compared with females. Correspondingly, the phloem-feeding males produced more frontalin than JHIII-treated males, and the production of frontalin was higher in paired males than in solitary males. The knockdown of mevalonate pathway genes using RNAi in vivo effectively reduced the messenger RNA level of these genes and inhibited the production of frontalin. Among them, the silencing of HMGR or HMGS genes reduced the synthesis of frontalin most significantly" |
| Keywords: | "Animals Bridged Bicyclo Compounds, Heterocyclic/*metabolism Female Male Mevalonic Acid/*metabolism Pheromones/*biosynthesis RNA Interference Weevils/genetics/*metabolism Chinese white pine beetle RNAi frontalin juvenile hormone (JH) III mevalonate pathway;" |
| Notes: | "MedlineSun, Yaya Fu, Danyang Kang, Xiaotong Liu, Bin Ning, Hang Chen, Hui eng 31870636/National Natural Science Foundation of China/ 2017YFD0600104/National Key Research and Development Program of China/ 2021/06/27 Arch Insect Biochem Physiol. 2021 Aug; 107(4):e21828. doi: 10.1002/arch.21828. Epub 2021 Jun 25" |
