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Front Physiol

Title:		Identification and Expression Patterns of Anoplophora chinensis (Forster) Chemosensory Receptor Genes from the Antennal Transcriptome
Author(s):		Sun L; Zhang YN; Qian JL; Kang K; Zhang XQ; Deng JD; Tang YP; Chen C; Hansen L; Xu T; Zhang QH; Zhang LW;
Address:		"Anhui Provincial Key Laboratory of Microbial Control, School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei, China. College of Life Sciences, Huaibei Normal University, Huaibei, China. Forest Diseases and Insect Pests Control and Quarantine Station of Chaohu City, Chaohu, China. College of Environmental Science and Forestry, State University of New York, Syracuse, NY, United States. Sterling International, Inc., Spokane, WA, United States"
Journal Title:		Front Physiol
Year:		2018
Volume:		20180213
Issue:
Page Number:		90 -
DOI:		10.3389/fphys.2018.00090
ISSN/ISBN:		1664-042X (Print) 1664-042X (Electronic) 1664-042X (Linking)
Abstract:		"The citrus long-horned beetle (CLB), Anoplophora chinensis (Forster) is a destructive native pest in China. Chemosensory receptors including odorant receptors (ORs), gustatory receptors (GRs), and ionotropic receptors (IRs) function to interface the insect with its chemical environment. In the current study, we assembled the antennal transcriptome of A. chinensis by next-generation sequencing. We assembled 44,938 unigenes from 64,787,784 clean reads and annotated their putative gene functions based on gene ontology (GO) and Clusters of Orthologous Groups of proteins (COG). Overall, 74 putative receptor genes from chemosensory receptor gene families, including 53 ORs, 17 GRs, and 4 IRs were identified. Expression patterns of these receptors on the antennae, maxillary and labial palps, and remaining body segments of both male and female A. chinensis were performed using quantitative real time-PCR (RT-qPCR). The results revealed that 23 ORs, 6 GRs, and 1 IR showed male-biased expression profiles, suggesting that they may play a significant role in sensing female-produced sex pheromones; whereas 8 ORs, 5 GRs, and 1 IR showed female-biased expression profiles, indicating that these receptors may be involved in some female-specific behaviors such as oviposition site seeking. These results lay a solid foundation for deeply understanding CLB olfactory processing mechanisms. Moreover, by comparing our results with those from chemosensory receptor studies in other cerambycid species, several highly probable pheromone receptor candidates were highlighted, which may facilitate the identification of additional pheromone and/or host attractants in CLB"
Keywords:		Anoplophora chinensis antennal transcriptome expression pattern gustatory receptor ionotropic receptor odorant receptor;
Notes:		"PubMed-not-MEDLINESun, Long Zhang, Ya-Nan Qian, Jia-Li Kang, Ke Zhang, Xiao-Qing Deng, Jun-Dan Tang, Yan-Ping Chen, Cheng Hansen, Laura Xu, Tian Zhang, Qing-He Zhang, Long-Wa eng Switzerland 2018/03/03 Front Physiol. 2018 Feb 13; 9:90. doi: 10.3389/fphys.2018.00090. eCollection 2018"