| Title: | "Developmental expression of FXPRLamide neuropeptides in peptidergic neurosecretory cells of diapause- and nondiapause-destined individuals of the cotton bollworm, Helicoverpa armigera" |
| Author(s): | Sun JS; Zhang QR; Zhang TY; Zhu ZL; Zhang HM; Teng MK; Niu LW; Xu WH; |
| Address: | "Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China" |
| DOI: | 10.1016/j.ygcen.2004.11.015 |
| ISSN/ISBN: | 0016-6480 (Print) 0016-6480 (Linking) |
| Abstract: | "The diapause hormone (DH)-pheromone biosynthesis activating neuropeptide (PBAN) gene encodes five neuropeptides, DH, PBAN, alpha-SGNP, beta-SGNP, and gamma-SGNP (subesophageal ganglion neuropeptide). All share the C-terminal pentapeptide FXPRLamide sequence and are produced in the subesophageal ganglion (SG). Expression of the DH-PBAN gene in the central nervous system of embryonic, larval, pupal, and adult Helicoverpa armigera (Har) was studied using in situ hybridization, whole-mount immunocytochemistry, and competitive ELISA. Both Har-DH-PBAN mRNA and protein are localized in the mandibular, maxillary, and labial cell clusters of the SG and a pair of ventral midline neurons of each thoracic ganglion. The FXPRLamide titers in hemolymph are significantly higher in diapause-destined larvae during the fifth and sixth instar than in similar nondiapause-destined individuals. In contrast, the FXPRLamide titers in diapause-destined pupae are significantly lower than in nondiapause-destined pupae. The results from immunocytochemistry and in situ hybridization are consistent with changes of FXPRLamide titers as measured by ELISA. These data suggest that the expression of DH-PBAN might be correlated with diapause induction at the larval stage of diapause-destined individuals and continuous development at pupal stage of nondiapause-destined individuals. Thus, the DH-PBAN gene may play an important regulatory role in aspects of insect development besides diapause termination and pheromone biosynthesis. The transport pathways of FXPRLamide neuropeptides suggest that humoral route is involved in their regulation of development" |
| Keywords: | "Animals Enzyme-Linked Immunosorbent Assay *Gene Expression Regulation, Developmental Hemolymph Immunohistochemistry In Situ Hybridization Larva/growth & development Moths/*genetics/*growth & development Neuropeptides/*biosynthesis/genetics;" |
| Notes: | "MedlineSun, Jiu-Song Zhang, Qi-Rui Zhang, Tian-Yi Zhu, Zhong-Liang Zhang, Hong-Min Teng, Mai-Kun Niu, Li-Wen Xu, Wei-Hua eng Research Support, Non-U.S. Gov't 2005/02/15 Gen Comp Endocrinol. 2005 Mar; 141(1):48-57. doi: 10.1016/j.ygcen.2004.11.015. Epub 2005 Jan 18" |
