| Title: | "Knockout of the odorant receptor co-receptor, orco, impairs feeding, mating and egg-laying behavior in the fall armyworm Spodoptera frugiperda" |
| Author(s): | Sun H; Bu LA; Su SC; Guo D; Gao CF; Wu SF; |
| Address: | "College of Plant Protection, Nanjing Agricultural University, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Weigang Road 1, Nanjing, 210095, Jiangsu, China. College of Plant Protection, Nanjing Agricultural University, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Weigang Road 1, Nanjing, 210095, Jiangsu, China. Electronic address: wusf@njau.edu.cn" |
| DOI: | 10.1016/j.ibmb.2022.103889 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "The olfactory transduction system of insects is involved in multiple behavioral processes such as foraging, mating, and egg-laying behavior. In the insect olfactory receptor neurons (ORNs), the odorant receptor co-receptor (Orco) is an obligatory component that is required for dimerization with odorant receptors (ORs) to form a ligand-gated ion channel complex. The ORs/Orco heteromeric complex plays a crucial role in insect olfaction. To explore the function of OR-mediated olfaction in the physiological behavior of the fall armyworm, Spodoptera frugiperda, we applied CRISPR/Cas9 genome editing to mutate its Orco gene and constructed a homozygous mutant strain of Orco (Orco(-/-)) by genetic crosses. Electroantennogram (EAG) analysis showed that the responses of Orco(-/-) male moths to two universal sex pheromones, Z9-14: Ac and Z7-12: Ac, were abolished. We found that Orco(-/-) males cannot successfully mate with female moths. An oviposition preference assay confirmed that Orco(-/-) female moths had a reduced preference for the optimal host plant maize. A larval feeding assay revealed that the time for Orco(-/-) larvae to locate the food source was significantly longer than in the wild-type. Overall, in the absence of Orco, the OR-dependent olfactory behavior was impaired in both larval and adult stages. Our results confirm that Orco is essential for multiple behavioral processes related to olfaction in the fall armyworm" |
| Keywords: | "Male Female Animals *Receptors, Odorant/genetics Spodoptera/genetics Oviposition Smell/genetics *Olfactory Receptor Neurons *Moths Larva/genetics Insecta CRISPR/Cas9 Egg laying behavior Insect olfactory Orco Spodoptera frugiperda;" |
| Notes: | "MedlineSun, Hao Bu, Ling-Ao Su, Shao-Cong Guo, Di Gao, Cong-Fen Wu, Shun-Fan eng Research Support, Non-U.S. Gov't England 2022/12/10 Insect Biochem Mol Biol. 2023 Jan; 152:103889. doi: 10.1016/j.ibmb.2022.103889. Epub 2022 Dec 7" |
