| Title: | Jasmonate-Inducible R2R3-MYB Transcription Factor Regulates Capsaicinoid Biosynthesis and Stamen Development in Capsicum |
| Author(s): | Sun B; Zhu Z; Chen C; Chen G; Cao B; Chen C; Lei J; |
| Address: | "Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in South China, Ministry of Agriculture, College of Horticulture , South China Agricultural University , Guangzhou , Guangdong 510642 , People's Republic of China. Henry School of Agricutural Science and Engineering , Shaoguang University , Guangdong 512005 , People's Republic of China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Jasmonates (JAs) play an important role in plant developmental processes and regulate the biosynthesis of various specialized metabolites, and transcription factors are crucial in mediating JA signaling to regulate these processes. Capsaicinoids (Caps) are intriguing specialized metabolites produced uniquely by Capsicum species that give their fruits a pungent flavor to defend against herbivory and pathogens. In this study, we identify a R2R3-MYB transcription factor CaMYB108 and demonstrate its roles in regulating the biosynthesis of Caps and stamen development. Transcriptional analysis indicated that CaMYB108 was preferentially expressed in the flower and fruit, while the subcellular localization of CaMYB108 was shown to be the nucleus. Virus-induced gene silencing of CaMYB108 led to the expression of capsaicinoid biosynthetic genes (CBGs), and the contents of Caps dramatically reduce. Moreover, the CaMYB108-silenced plants showed delayed anther dehiscence and reduced pollen viability. Transient overexpression of CaMYB108 caused the expression of CBGs to be upregulated, and the Caps content significantly increased. The results of dual-luciferase reporter assays showed that CaMYB108 targeted CBG promoters. In addition, the expression of CaMYB108 and CBGs was inducible by methyl jasmonate and was consistent with the increased content of Caps. Overall, our results indicate that CaMYB108 is involved in the regulation of Caps biosynthesis and stamen development" |
| Keywords: | "Capsaicin/*metabolism Capsicum/genetics/growth & development/*metabolism Cyclopentanes/*metabolism Flowers/genetics/*growth & development/metabolism Gene Expression Regulation, Plant Oxylipins/*metabolism Plant Proteins/genetics/*metabolism Pollen/genetic;" |
| Notes: | "MedlineSun, Binmei Zhu, Zhangsheng Chen, Chengjie Chen, Guoju Cao, Bihao Chen, Changming Lei, Jianjun eng 2019/09/12 J Agric Food Chem. 2019 Oct 2; 67(39):10891-10903. doi: 10.1021/acs.jafc.9b04978. Epub 2019 Sep 20" |
