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Sci Rep

Title:		The histone H2B Arg95 residue efficiently recruits the transcription factor Spt16 to mediate Ste5 expression of the pheromone response pathway
Author(s):		Sulaiman AA; Ali R; Ramotar D;
Address:		"Division of Biological and Biomedical Sciences, College of Health and Life Sciences, Hamad Bin Khalifa University, Qatar Foundation, Education City, P.O. Box: 34110, Doha, Qatar. Division of Biological and Biomedical Sciences, College of Health and Life Sciences, Hamad Bin Khalifa University, Qatar Foundation, Education City, P.O. Box: 34110, Doha, Qatar. dramotar@hbku.edu.qa"
Journal Title:		Sci Rep
Year:		2023
Volume:		20230622
Issue:		1
Page Number:		10189 -
DOI:		10.1038/s41598-023-37339-y
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin inhibits the TORC1 kinase causing rapid alteration in gene expression and leading to G(1) arrest. We recently reported the isolation and characterization from the histone mutant collection of a histone H2B R95A mutant that displays resistance to rapamycin. This mutant is defective in the expression of several genes belonging to the pheromone response pathway including STE5 encoding a scaffold protein that promotes the activation of downstream MAP kinases. Cells lacking Ste5 cannot arrest the cell cycle in response to rapamycin and as a consequence exhibit similar resistance to rapamycin as the H2B R95A mutant. Herein, we show that the H2B R95A mutation weakens the association of H2B with Spt16 a component of the FACT complex (FAcilitates Chromatin Transcription), and an essential factor that interacts with the histone H2A-H2B dimer to promote transcription and preserve chromatin integrity. From a collection of spt16 mutants, spt16 E857K and spt16-11 showed striking sensitivity to rapamycin as compared to the parent strain. spt16 E857K and spt16-11 expressed distinct forms of Ste5, while a suppressor mutation H2B A84D of the spt16-11 mutant prevents the expression of Ste5 and confers marked resistance to rapamycin. We interpret these findings to suggest that the Arg95 residue of histone H2B is required to recruit Spt16 to maintain the expression of STE5, which performs a role to arrest cells in the G(1) phase in response to rapamycin"
Keywords:		*Histones/genetics/metabolism Transcription Factors/genetics/metabolism Nucleosomes/metabolism *Saccharomyces cerevisiae Proteins/genetics/metabolism Transcriptional Elongation Factors/metabolism Chromatin/metabolism Saccharomyces cerevisiae/genetics/meta;
Notes:		"MedlineSulaiman, Abdallah Alhaj Ali, Reem Ramotar, Dindial eng England 2023/06/23 Sci Rep. 2023 Jun 22; 13(1):10189. doi: 10.1038/s41598-023-37339-y"