| Title: | FEV manoeuvre induced changes in breath VOC compositions: an unconventional view on lung function tests |
| Author(s): | Sukul P; Schubert JK; Oertel P; Kamysek S; Taunk K; Trefz P; Miekisch W; |
| Address: | "Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Department of Anaesthesiology and Intensive Care, Rostock University Medical Center, Schillingallee 35, Rostock, 18057, Germany. Proteomics Lab, National Centre for Cell Science (NCCS), Pune University, Pune (Maharashtra), 411007, India" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Breath volatile organic compound (VOC) analysis can open a non-invasive window onto pathological and metabolic processes in the body. Decades of clinical breath-gas analysis have revealed that changes in exhaled VOC concentrations are important rather than disease specific biomarkers. As physiological parameters, such as respiratory rate or cardiac output, have profound effects on exhaled VOCs, here we investigated VOC exhalation under respiratory manoeuvres. Breath VOCs were monitored by means of real-time mass-spectrometry during conventional FEV manoeuvres in 50 healthy humans. Simultaneously, we measured respiratory and hemodynamic parameters noninvasively. Tidal volume and minute ventilation increased by 292 and 171% during the manoeuvre. FEV manoeuvre induced substance specific changes in VOC concentrations. pET-CO2 and alveolar isoprene increased by 6 and 21% during maximum exhalation. Then they decreased by 18 and 37% at forced expiration mirroring cardiac output. Acetone concentrations rose by 4.5% despite increasing minute ventilation. Blood-borne furan and dimethyl-sulphide mimicked isoprene profile. Exogenous acetonitrile, sulphides, and most aliphatic and aromatic VOCs changed minimally. Reliable breath tests must avoid forced breathing. As isoprene exhalations mirrored FEV performances, endogenous VOCs might assure quality of lung function tests. Analysis of exhaled VOC concentrations can provide additional information on physiology of respiration and gas exchange" |
| Keywords: | Acetone/analysis/toxicity Adult Butadienes/analysis/toxicity Carbon Dioxide/chemistry/metabolism Exhalation Female Forced Expiratory Volume/drug effects/*physiology Hemiterpenes/analysis/toxicity Hemodynamics/physiology Humans Male Mass Spectrometry Middl; |
| Notes: | "MedlineSukul, Pritam Schubert, Jochen K Oertel, Peter Kamysek, Svend Taunk, Khushman Trefz, Phillip Miekisch, Wolfram eng Research Support, Non-U.S. Gov't England 2016/06/18 Sci Rep. 2016 Jun 17; 6:28029. doi: 10.1038/srep28029" |
