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Insect Biochem Mol Biol

Title:		Acp70A regulates Drosophila pheromones through juvenile hormone induction
Author(s):		Bontonou G; Shaik HA; Denis B; Wicker-Thomas C;
Address:		"CNRS UPR 9034, Universite de Paris Sud, 91198 Gif sur Yvette, France. CNRS UPR 9034, Universite de Paris Sud, 91198 Gif sur Yvette, France. Electronic address: Claude.Wicker-thomas@legs.cnrs-gif.fr"
Journal Title:		Insect Biochem Mol Biol
Year:		2015
Volume:		20141204
Issue:
Page Number:		36 - 49
DOI:		10.1016/j.ibmb.2014.11.008
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Mated Drosophila melanogaster females show a decrease in mating receptivity, enhanced ovogenesis, egg-laying and activation of juvenile hormone (JH) production. Components in the male seminal fluid, especially the sex peptide ACP70A stimulate these responses in females. Here we demonstrate that ACP70A is involved in the down-regulation of female sex pheromones and hydrocarbon (CHC) production. Drosophila G10 females which express Acp70A under the control of the vitellogenin gene yp1, produced fewer pheromones and CHCs. There was a dose-dependent relationship between the number of yp1-Acp70A alleles and the reduction of these compounds. Similarly, a decrease in CHCs and diene pheromones was observed in da > Acp70A flies that ubiquitously overexpress Acp70A. Quantitative-PCR experiments showed that the expression of Acp70A in G10 females was the same as in control males and 5 times lower than in da > Acp70A females. Three to four days after injection with 4.8 pmol ACP70A, females from two different strains, exhibited a significant decrease in CHC and pheromone levels. Similar phenotypes were observed in ACP70A injected flies whose ACP70A receptor expression was knocked-down by RNAi and in flies which overexpress ACP70A N-terminal domain. These results suggest that the action of ACP70A on CHCs could be a consequence of JH activation. Female flies exposed to a JH analog had reduced amounts of pheromones, whereas genetic ablation of the corpora allata or knock-down of the JH receptor Met, resulted in higher amounts of both CHCs and pheromonal dienes. Mating had negligible effects on CHC levels, however pheromone amounts were slightly reduced 3 and 4 days post copulation. The physiological significance of ACP70A on female pheromone synthesis is discussed"
Keywords:		Animals Copulation/physiology Corpora Allata/metabolism Down-Regulation Drosophila Proteins/*pharmacology Drosophila melanogaster/genetics/*metabolism/*physiology Female Hydrocarbons/metabolism Juvenile Hormones/*metabolism Male Peptides/*pharmacology Phe;
Notes:		"MedlineBontonou, Gwenaelle Shaik, Haq Abdul Denis, Beatrice Wicker-Thomas, Claude eng Research Support, Non-U.S. Gov't England 2014/12/09 Insect Biochem Mol Biol. 2015 Jan; 56:36-49. doi: 10.1016/j.ibmb.2014.11.008. Epub 2014 Dec 4"