« Previous AbstractAre odorant-binding proteins involved in odorant discrimination?    Next AbstractHerbivore-induced changes in tomato (Solanum lycopersicum) primary metabolism: a whole plant perspective »

Methods Enzymol

Title:		Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins
Author(s):		Steinbrecht RA;
Address:		"Max-Planck-Institute for Ornithology, Seewiesen, Germany. Electronic address: steinbre@orn.mpg.de"
Journal Title:		Methods Enzymol
Year:		2020
Volume:		20200522
Issue:
Page Number:		259 - 278
DOI:		10.1016/bs.mie.2020.04.064
ISSN/ISBN:		1557-7988 (Electronic) 0076-6879 (Linking)
Abstract:		Fine structure immunocytochemistry enables the in situ localization of odorant-binding proteins with the high resolution of the electron microscope. Protocols for successfully labeling these proteins in insect chemosensory sensilla are given and some pitfalls pinpointed as well as ways to avoid them. The major achievements accomplished by these methods are briefly reviewed
Keywords:		Animals Carrier Proteins Immunohistochemistry *Insect Proteins/metabolism *Odorants Sensilla/metabolism Chemosensory sensilla Electron microscopy Immunolabeling Odorant-binding protein Pheromone-binding protein;
Notes:		"MedlineSteinbrecht, Rudolf Alexander eng 2020/08/24 Methods Enzymol. 2020; 642:259-278. doi: 10.1016/bs.mie.2020.04.064. Epub 2020 May 22"