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Molecules

Title:		Hechtia glomerata Zucc: Phytochemistry and Activity of Its Extracts and Major Constituents Against Resistant Bacteria
Author(s):		Stefani T; Garza-Gonzalez E; Rivas-Galindo VM; Rios MY; Alvarez L; Camacho-Corona MDR;
Address:		"Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad S/N, Ciudad Universitaria, CP San Nicolas de los Garza 66451, Nuevo Leon, Mexico. tstefani854@gmail.com. Universidad Autonoma de Nuevo Leon, Servicio de Gastroenterologia Hospital Universitario Dr. Jose Eleuterio Gonzalez, Av. Gonzalitos y Madero S/N, Col. Mitras Centro, CP Monterrey 64460, Nuevo Leon, Mexico. elvira_garza_gzz@yahoo.com. Universidad Autonoma de Nuevo Leon, Facultad de Medicina, Av. Madero S/N, Col. Mitras Centro, CP Monterrey 64460, Nuevo Leon, Mexico. veronica.rivasgl@uanl.edu.mx. Universidad Autonoma del Estado de Morelos, Centro de Investigaciones Quimicas IICBA, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico. myolanda@uaem.mx. Universidad Autonoma del Estado de Morelos, Centro de Investigaciones Quimicas IICBA, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico. lalvarez@uaem.mx. Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, Av. Universidad S/N, Ciudad Universitaria, CP San Nicolas de los Garza 66451, Nuevo Leon, Mexico. maria.camachocn@uanl.edu.mx"
Journal Title:		Molecules
Year:		2019
Volume:		20190921
Issue:		19
Page Number:		-
DOI:		10.3390/molecules24193434
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Hechtia glomerata Zucc. is used both as a source of food and in ethnomedicine to treat various diseases derived from bacterial infections such as bronchitis, laryngitis, nephritis, whooping cough, urethritis, and sepsis. There are no previous reports about its chemistry and biological activities. Therefore, the aims of this study were to identify components from organic and aqueous extracts of H. glomerata and test the extracts and major isolate compounds against resistant bacteria. Hexane, CHCl(3)/MeOH, and aqueous extracts were prepared and analyzed by different chromatographic techniques. Structural elucidation was carried out by NMR spectroscopy and X-ray diffraction. The antibacterial activities of extracts, phytochemicals, and semisynthetic derivatives against resistant bacteria were determined by the broth micro-dilution method. From the hexane extract nonacosane (1), hexatriacontanyl stearate (2), hexacosanol (3), oleic acid (4), and beta-sitosterol (5) were isolated and characterized. From the CHCl(3)/MeOH extract, p-coumaric acid (6), margaric acid (7), caffeic acid (8), daucosterol (9), and potassium chloride (10) were isolated and characterized. A total of 58 volatile compounds were identified by GC-MS from the hexane extract and two solids were isolated from the CHCl(3)/MeOH extract. The UPLC-QTOF-MS analysis of the aqueous extract allowed the identification of 55 polar compounds. Hexane and aqueous extracts showed antibacterial activity against ESBL Escherichia coli, and three strains of Klebsiella pneumoniae ESBL, NDM-1 +, and OXA-48 with MIC values of 500 microg/mL. The CHCl(3)/MeOH extract was devoid of activity. The activity of phytocompounds and their semisynthetic derivatives toward resistant bacteria was weak. The most active compound was beta-sitosterol acetate, with a MIC value of 100 microg/mL against carbapenem-resistant A. baumannii. This is the first report of the secondary metabolites of H. glomerata Zucc. and the activity of its extracts and major pure compounds against resistant bacterial strains"
Keywords:		"Alkanes/chemistry Bacteria/*drug effects Escherichia coli/drug effects Klebsiella pneumoniae/drug effects Medicine, Traditional Microbial Sensitivity Tests Oleic Acid/chemistry Phytochemicals/chemistry/*pharmacology Plant Extracts/chemistry Sitosterols/ch;"
Notes:		"MedlineStefani, Tommaso Garza-Gonzalez, Elvira Rivas-Galindo, Veronica M Rios, Maria Yolanda Alvarez, Laura Camacho-Corona, Maria Del Rayo eng Switzerland 2019/09/25 Molecules. 2019 Sep 21; 24(19):3434. doi: 10.3390/molecules24193434"