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J AOAC Int


Title:Fingerprinting of the volatile fraction from selected thyme species by means of headspace gas chromatography with mass spectrometric detection
Author(s):Staszek D; Orlowska M; Rzepa J; Wrobel MS; Kowalska T; Szymczak G; Waksmundzka-Hajnos M;
Address:"University of Silesia, Institute of Chemistry, 9 Szkolna St, Katowice, Poland"
Journal Title:J AOAC Int
Year:2014
Volume:97
Issue:5
Page Number:1250 - 1258
DOI: 10.5740/jaoacint.SGEStaszek
ISSN/ISBN:1944-7922 (Electronic) 1060-3271 (Linking)
Abstract:"The Thymus genus has a firm position in Mediterranean folk medicine and culinary tradition, and yet certain confusion is observed in its botanical taxonomy. Therefore, chemical analysis of secondary metabolites and selection of reliable chemotaxonomic markers can prove helpful. In this study, the volatile fraction derived from 20 different thyme specimens was analyzed by means of headspace GC with MS detection. From the obtained chromatographic fingerprints, the diversity of the volatile fraction originating from the different thyme species clearly emerged. Identification of volatile components was based on a software library of mass spectra. There was only one common component derived from all 20 investigated thyme species, i.e., ss-linalool. For three different Thymus species, a comparison was made of the performance of the headspace extraction and hydrodistillation. Characteristic differences in composition of the volatile fraction obtained with use of these two techniques were discussed. Finally, the obtained chromatographic fingerprints of the volatile fraction were analyzed by means of a chemometric approach (principal component analysis), in order to trace similarities in their chemical composition"
Keywords:Gas Chromatography-Mass Spectrometry/*methods Thymus Plant/*chemistry Volatile Organic Compounds/*analysis;
Notes:"MedlineStaszek, Dorota Orlowska, Marta Rzepa, Jozef Wrobel, Michal S Kowalska, Teresa Szymczak, Grazyna Waksmundzka-Hajnos, Monika eng England 2015/04/24 J AOAC Int. 2014 Sep-Oct; 97(5):1250-8. doi: 10.5740/jaoacint.SGEStaszek"

 
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