| Title: | Bacteria associated with truffle-fruiting bodies contribute to truffle aroma |
| Author(s): | Splivallo R; Deveau A; Valdez N; Kirchhoff N; Frey-Klett P; Karlovsky P; |
| Address: | "Molecular Phytopathology and Mycotoxin Research, Georg-August University of Goettingen, Goettingen, Germany. Institute for Molecular Biosciences, Biozentrum/Campus Riedberg, Frankfurt am Main, Germany. INRA, UMR 1136 INRA Universite de Lorraine 'Interactions Arbres/Micro-organismes', Champenoux, France" |
| ISSN/ISBN: | 1462-2920 (Electronic) 1462-2912 (Linking) |
| Abstract: | "Truffles, symbiotic fungi renown for the captivating aroma of their fruiting bodies, are colonized by a complex bacterial community of unknown function. We characterized the bacterial community of the white truffle Tuber borchii and tested the involvement of its microbiome in the production of sulphur-containing volatiles. We found that sulphur-containing volatiles such as thiophene derivatives, characteristic of T. borchii fruiting bodies, resulted from the biotransformation of non-volatile precursor(s) into volatile compounds by bacteria. The bacterial community of T. borchii was dominated by alpha- and beta-Proteobacteria. Interestingly, all bacteria phyla/classes tested in this study were able to produce thiophene volatiles from T. borchii fruiting body extract, irrespective of their isolation source (truffle or other sources). This indicates that the ability to produce thiophene volatiles might be widespread among bacteria and possibly linked to primary metabolism. Treatment of fruiting bodies with antibacterial agents fully suppressed the production of thiophene volatiles while fungicides had no inhibitory effect. This suggests that during the sexual stage of truffles, thiophene volatiles are exclusively synthesized by bacteria and not by the truffle. At this stage, the origin of thiophenes precursor in T. borchii remains elusive and the involvement of yeasts or other bacteria cannot be excluded" |
| Keywords: | "Alphaproteobacteria/isolation & purification/metabolism Ascomycota/*metabolism Betaproteobacteria/isolation & purification/metabolism Fruiting Bodies, Fungal/*metabolism Microbiota/*physiology Molecular Sequence Data Odorants Symbiosis Thiophenes/*metabol;" |
| Notes: | "MedlineSplivallo, Richard Deveau, Aurelie Valdez, Nayuf Kirchhoff, Nina Frey-Klett, Pascale Karlovsky, Petr eng Research Support, Non-U.S. Gov't England 2014/06/07 Environ Microbiol. 2015 Aug; 17(8):2647-60. doi: 10.1111/1462-2920.12521. Epub 2014 Jul 9" |
