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J Chem Ecol

Title:		Analysis of volatile organic compounds in human saliva by a static sorptive extraction method and gas chromatography-mass spectrometry
Author(s):		Soini HA; Klouckova I; Wiesler D; Oberzaucher E; Grammer K; Dixon SJ; Xu Y; Brereton RG; Penn DJ; Novotny MV;
Address:		"Institute for Pheromone Research and Department of Chemistry, Indiana University, 800 E Kirkwood Ave., Bloomington, IN 47405, USA"
Journal Title:		J Chem Ecol
Year:		2010
Volume:		20100831
Issue:		9
Page Number:		1035 - 1042
DOI:		10.1007/s10886-010-9846-7
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"Human saliva not only helps control oral health (with anti-microbial proteins), but it may also play a role in chemical communication. As is the case with other mammalian species, human saliva contains peptides, proteins, and numerous volatile organic compounds (VOCs). A high-throughput analytical method is described for profiling a large number of saliva samples to screen the profiles of VOCs. Saliva samples were collected in a non-stimulated fashion. The method utilized static stir bar extraction followed by gas chromatography-mass spectrometry (GC-MS). The method provided excellent reproducibility for a wide range of salivary compounds, including alcohols, aldehydes, ketones, carboxylic acids, esters, amines, amides, lactones, and hydrocarbons. Furthermore, substantial overlap of salivary VOCs and the previously reported skin VOCs in the same subject group was found in this study by using pattern recognition analyses. Sensitivity, precision, and reproducibility of the method suggest that this technique has potential in physiological, metabolomic, pharmacokinetic, forensic, and toxicological studies of small organic compounds where a large number of human saliva samples are involved"
Keywords:		Chemical Fractionation/*methods Female Gas Chromatography-Mass Spectrometry/*methods Humans Male Organic Chemicals/*analysis/chemistry/*isolation & purification Reproducibility of Results Saliva/*chemistry Volatilization;
Notes:		"MedlineSoini, Helena A Klouckova, Iveta Wiesler, Donald Oberzaucher, Elisabeth Grammer, Karl Dixon, Sarah J Xu, Yun Brereton, Richard G Penn, Dustin J Novotny, Milos V eng Research Support, Non-U.S. Gov't 2010/09/03 J Chem Ecol. 2010 Sep; 36(9):1035-42. doi: 10.1007/s10886-010-9846-7. Epub 2010 Aug 31"