| Title: | A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry |
| Author(s): | Smith S; Burden H; Persad R; Whittington K; de Lacy Costello B; Ratcliffe NM; Probert CS; |
| Address: | "Centre for Research in Analytical, Materials, and Sensor Sciences, University of the West of England, Bristol, BS16 1QY, UK" |
| DOI: | 10.1088/1752-7155/2/3/037022 |
| ISSN/ISBN: | 1752-7155 (Print) 1752-7155 (Linking) |
| Abstract: | "First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed" |
| Notes: | "PubMed-not-MEDLINESmith, S Burden, H Persad, R Whittington, K de Lacy Costello, B Ratcliffe, N M Probert, C S eng England 2008/09/01 J Breath Res. 2008 Sep; 2(3):037022. doi: 10.1088/1752-7155/2/3/037022. Epub 2008 Sep 8" |
