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Insect Biochem Mol Biol

Title:		Antennal expressed genes of the yellow fever mosquito (Aedes aegypti L.); characterization of odorant-binding protein 10 and takeout
Author(s):		Bohbot J; Vogt RG;
Address:		"Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA. jonathan@biol.sc.edu"
Journal Title:		Insect Biochem Mol Biol
Year:		2005
Volume:		35
Issue:		9
Page Number:		961 - 979
DOI:		10.1016/j.ibmb.2005.03.010
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"A small cDNA library was constructed from antennae of 100 adult male Aedes aegypti yellow fever mosquitoes. Sequencing of 80 clones identified 49 unique gene products, including a member of the Odorant Binding Protein family (Aaeg-OBP10), a homologue of Takeout (Aaeg-TO), and transposable elements of the LINE, SINE and MITE classes. Aaeg-OBP10 encodes a 140 amino acid protein including a predicted 25 amino acid signal peptide. Aaeg-OBP10 expression was adult male enriched, increased with adult age, and greatest in antennae and wings but also present in maxillary palps, proboscis and leg. Aaeg-OBP10 is a likely orthologue of Agam-OBP10 of the malaria mosquito Anopheles gambiae and shares significant similarity with members of the OBP56 gene cluster of Drosophila melanogaster. These OBP genes may represent a unified class of OBPs with unique roles in chemodetection; the expression pattern of Aaeg-OBP10 suggests it may play a role in adult male chemosensory behavior. Aaeg-TO encodes a 248 amino acid protein including a predicted 22 amino acid signal peptide. Aaeg-TO is homologous with the circadian/feeding regulated D. melanogaster Takeout protein (Dmel-TO) and a subclass of Juvenile Hormone Binding Proteins (JHBP) characterized by Moling from Manduca sexta; both Dmel-TO and Moling are sensitive to feeding, suggesting Aaeg-TO might regulate the antennal response to food, host or pheromonal odors in a JH sensitive manner. Aaeg-TO was used to identify 25 D. melanogaster and 13 A. gambiae homologues by Blast analysis suggesting these may comprise a relatively large class of protein involved in the hormonal regulation of behavior"
Keywords:		Aedes/genetics/*physiology Amino Acid Sequence Animals Base Sequence Expressed Sequence Tags Female Gene Expression Regulation/physiology Gene Library Insect Proteins/biosynthesis/*genetics/physiology Larva/metabolism Male Molecular Sequence Data Pheromon;
Notes:		"MedlineBohbot, Jonathan Vogt, Richard G eng Research Support, U.S. Gov't, Non-P.H.S. England 2005/06/28 Insect Biochem Mol Biol. 2005 Sep; 35(9):961-79. doi: 10.1016/j.ibmb.2005.03.010"