| Title: | A new enrichment approach identifies genes that alter cell cycle progression in Saccharomyces cerevisiae |
| Author(s): | Bogomolnaya LM; Pathak R; Cham R; Guo J; Surovtseva YV; Jaeckel L; Polymenis M; |
| Address: | "Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA" |
| DOI: | 10.1007/s00294-004-0497-5 |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "Mechanisms that coordinate cell growth with division are thought to determine the timing of initiation of cell division and to limit overall cell proliferation. To identify genes involved in this process in Saccharomyces cerevisiae, we describe a method that does not rely on cell size alterations or resistance to pheromone. Instead, our approach was based on the cell surface deposition of the Flo1p protein in cells having passed START. We found that over-expression of HXT11 (which encodes a plasma membrane transporter), PPE1 (coding for a protein methyl esterase), or SIK1 (which encodes a protein involved in rRNA processing) shortened the duration of the G1 phase of the cell cycle, prior to the initiation of DNA replication. In addition, we found that, although SIK1 was not part of a mitotic checkpoint, SIK1 over-expression caused spindle orientation defects and sensitized G2/M checkpoint mutant cells. Thus, unlike HXT11 and PPE1, SIK1 over-expression is also associated with mitotic functions. Overall, we used a novel enrichment approach and identified genes that were not previously associated with cell cycle progression. This approach can be extended to other organisms" |
| Keywords: | "Cell Cycle Proteins/*genetics/metabolism Cloning, Molecular/*methods Gene Expression Regulation, Fungal/genetics/physiology Genes, Fungal Genomic Library Mannose-Binding Lectins Mitosis/*genetics/physiology Saccharomyces cerevisiae/*genetics/physiology Sa;" |
| Notes: | "MedlineBogomolnaya, Lydia M Pathak, Ritu Cham, Roxana Guo, Jinbai Surovtseva, Yulia V Jaeckel, Lane Polymenis, Michael eng R01 GM062377/GM/NIGMS NIH HHS/ GM062377/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2004/03/17 Curr Genet. 2004 Jun; 45(6):350-9. doi: 10.1007/s00294-004-0497-5. Epub 2004 Mar 12" |
