| Title: | A novel MAP-kinase kinase from Candida albicans |
| Author(s): | Singh P; Ghosh S; Datta A; |
| Address: | "School of Life Sciences, Jawaharlal Nehru University, New Delhi, India" |
| DOI: | 10.1016/s0378-1119(96)00758-5 |
| ISSN/ISBN: | 0378-1119 (Print) 0378-1119 (Linking) |
| Abstract: | "A putative MAP-kinase kinase-encoding gene, CaSTE7, was isolated from Candida albicans by complementation of ste7 and ste11 mutants of the pheromone signal-transduction pathway of Saccharomyces cerevisiae. The nucleotide (nt) sequence revealed an ORF of 1767 nt encoding a putative protein of 589 amino acids (aa). CaSTE7 has a strong homology with MAP-kinase kinase STE7 of S. cerevisiae, the kinase domain having 45% homology with that of STE7. The deduced aa sequence contained all eleven consensus kinase subdomains found in MAP-kinase kinases. It can suppress the mating defect of ste5, ste11, ste7, and fus3 kss1 double mutants, but it cannot bypass the ste12 mutation. CaSTE7 behaves as a hyperactive allele of STE7, suppressing the mating defects of the pheromone signal-transduction pathway by constitutively stimulating STE12, and hence STE12-dependent processes" |
| Keywords: | "Amino Acid Sequence Base Sequence Candida albicans/*enzymology DNA, Fungal Fungal Proteins/metabolism Mitogen-Activated Protein Kinase Kinases Molecular Sequence Data Protein Kinases/genetics/*isolation & purification/metabolism *Saccharomyces cerevisiae;" |
| Notes: | "MedlineSingh, P Ghosh, S Datta, A eng Research Support, Non-U.S. Gov't Netherlands 1997/04/29 Gene. 1997 Apr 29; 190(1):99-104. doi: 10.1016/s0378-1119(96)00758-5" |
