| Title: | "In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori" |
| Author(s): | Shiota Y; Sakurai T; Daimon T; Mitsuno H; Fujii T; Matsuyama S; Sezutsu H; Ishikawa Y; Kanzaki R; |
| Address: | "Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan. ts206448@nodai.ac.jp. Department of Agricultural Innovation for Sustainability, Faculty of Agriculture, Tokyo University of Agriculture, 1737 Funako, Atsugi, Kanagawa, 243-0034, Japan. ts206448@nodai.ac.jp. Department of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan. Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8567, Japan. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan. Transgenic Silkworm Research Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan" |
| DOI: | 10.1038/s41598-018-31978-2 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Male moths detect sex pheromones emitted by conspecific females with high sensitivity and specificity by the olfactory sensilla on their antennae. Pheromone binding proteins (PBPs) are highly enriched in the sensillum lymph of pheromone sensitive olfactory sensilla and are supposed to contribute to the sensitivity and selectivity of pheromone detection in moths. However, the functional role of PBPs in moth sex pheromone detection in vivo remains obscure. In the silkmoth, Bombyx mori, female moths emit bombykol as a single attractive sex pheromone component along with a small amount of bombykal that negatively modulates the behavioural responses to bombykol. A pair of olfactory receptor neurons, specifically tuned to bombykol or bombykal, co-localise in the trichodeum sensilla, the sensillum lymph of which contains a single PBP, namely, BmPBP1. We analysed the roles of BmPBP1 using BmPBP1-knockout silkmoth lines generated by transcription activator-like effector nuclease-mediated gene targeting. Electroantennogram analysis revealed that the peak response amplitudes of BmPBP1-knockout male antennae to bombykol and bombykal were significantly reduced by a similar percentage when compared with those of the wild-type males. Our results indicate that BmPBP1 plays a crucial role in enhancing the sensitivity, but not the selectivity, of sex pheromone detection in silkmoths" |
| Keywords: | "Alkadienes/metabolism Animals Animals, Genetically Modified Bombyx/*physiology Carrier Proteins/genetics/*metabolism Fatty Alcohols/metabolism Female Gene Knockdown Techniques Insect Proteins/genetics/*metabolism Male Olfactory Receptor Neurons/metabolism;" |
| Notes: | "MedlineShiota, Yusuke Sakurai, Takeshi Daimon, Takaaki Mitsuno, Hidefumi Fujii, Takeshi Matsuyama, Shigeru Sezutsu, Hideki Ishikawa, Yukio Kanzaki, Ryohei eng 26712027/Japan Society for the Promotion of Science (JSPS)/International 15H04399/Japan Society for the Promotion of Science (JSPS)/International Research Support, Non-U.S. Gov't England 2018/09/12 Sci Rep. 2018 Sep 10; 8(1):13529. doi: 10.1038/s41598-018-31978-2" |
