| Title: | Developmental regulation of the pheromone biosynthesis activating neuropeptide-receptor (PBAN-R): re-evaluating the role of juvenile hormone |
| Author(s): | Bober R; Azrielli A; Rafaeli A; |
| Address: | "Department of Entomology, The Hebrew University, Rehovot, Israel" |
| DOI: | 10.1111/j.1365-2583.2009.00937.x |
| ISSN/ISBN: | 1365-2583 (Electronic) 0962-1075 (Linking) |
| Abstract: | "Sex pheromone production in Helicoverpa armigera is regulated by pheromone-biosynthesis-activating neuropeptide (PBAN), which binds to a G-protein coupled receptor at the pheromone gland. We demonstrate the temporal differential expression levels of the PBAN receptor (PBAN-R) gene, reaching peak levels at a critical period of 5 h post-eclosion. Previous studies implied a possible regulatory role for juvenile hormone (JH). We herein demonstrate that PBAN-R expression levels increase normally when females are decapitated or head-ligated, removing the source of JH, before peak transcript levels are reached. Similarly, sex pheromone production can be induced by PBAN in such decapitated females. These results indicate that up-regulation, at this critical time, is not dependent on JH originating from the head. Conversely, JH injected in vivo at this critical period significantly inhibits PBAN-R transcript levels" |
| Keywords: | "Animals Decapitation Female Gene Expression Regulation, Developmental Insect Proteins/*metabolism Insecticides Juvenile Hormones/*metabolism Male Moths/genetics/*metabolism Neuropeptides/metabolism Phenylcarbamates Receptors, G-Protein-Coupled/*metabolism;" |
| Notes: | "MedlineBober, R Azrielli, A Rafaeli, A eng Comparative Study Research Support, Non-U.S. Gov't England 2009/12/17 Insect Mol Biol. 2010 Feb; 19(1):77-86. doi: 10.1111/j.1365-2583.2009.00937.x. Epub 2009 Dec 1" |
