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Environ Toxicol

Title:		Effect on the H19 gene methylation of sperm and organs of offspring after chlorpyrifos-methyl exposure during organogenesis period
Author(s):		Shin HS; Seo JH; Jeong SH; Park SW; Park YI; Son SW; Kang HG; Kim JS;
Address:		"Toxicology & Residue Chemistry Division, Animal, Plant and Fisheries Quarantine and Inspection Agency, 175 Anyangro, Manan-gu, Anyang-si, Gyeonggi-do 430-757, Republic of Korea. GLP Research Center, College of Natural Science, Hoseo University, 79 Hoseoro, Baebangup, Asan-si, Chungnam, 336-795, Republic of Korea. Pharmacology & Toxicology Laboratory, College of Veterinary Medicine, Konkuk University, 120 Neungdongro, Gwangjin-gu, Seoul, 143-701, Republic of Korea"
Journal Title:		Environ Toxicol
Year:		2015
Volume:		20150317
Issue:		12
Page Number:		1355 - 1363
DOI:		10.1002/tox.21923
ISSN/ISBN:		1522-7278 (Electronic) 1520-4081 (Linking)
Abstract:		"To elucidate the effect on the H19 gene methylation of sperm and organs in offspring by chlorpyrifos-methyl (CPM) exposure during organogenesis period, CPM was administered at doses of 4 (CPM4), 20 (CPM20), and 100 (CPM100) mg/kg bw/day from 7 days post coitum (d.p.c.) to 17 d.p.c. after mating CAST/Ei (male symbol) and B6 (female symbol). Anogenital distance (AGD) was measured at postnatal day (PND) 21. Clinical signs, body weights, feed and water consumption, organs weights, serum hormone values, and H19 methylation level of organ and sperm were measured at PND63. Body weights were significantly lower than control until PND6. AGD was significantly decreased in the CPM100 group in males and increased in the CPM20 group in females. The absolute weights of the thymus and epididymis were significantly increased for males in all of CPM treatment groups. In the CPM20 group, absolute weights of liver, kidney, heart, lung, spleen, prostate gland, and testes were significantly increased. Testosterone concentrations in serum were significantly increased by CPM treatment in males. H19 methylation level of liver and thymus showed decreased pattern in a dose-dependent manner in males. The levels of H19 methylation in sperm were 73.76 +/- 7.16% (Control), 57.84 +/- 12.94% (CPM4), 64.24 +/- 3.79% (CPM20), and 64.24 +/- 3.79% (CPM100). Conclusively, CPM exposure during organogenesis period can disrupt H19 methylation in sperm, liver, and thymus and disturb the early development of offspring"
Keywords:		"Animals Body Weight/drug effects Chlorpyrifos/*analogs & derivatives/toxicity CpG Islands DNA Methylation/*drug effects Enzyme-Linked Immunosorbent Assay Female Liver/metabolism Male Mice Mice, Inbred C57BL Organ Size/drug effects Organogenesis/*drug effe;"
Notes:		"MedlineShin, Hyo-Sook Seo, Jong-Hun Jeong, Sang-Hee Park, Sung-Won Park, Young-Il Son, Seong-Wan Kang, Hwan-Goo Kim, Jin Suk eng 2015/03/19 Environ Toxicol. 2015 Dec; 30(12):1355-63. doi: 10.1002/tox.21923. Epub 2015 Mar 17"