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J Cell Biochem

Title:		"N-terminal residues of the yeast pheromone receptor, Ste2p, mediate mating events independently of G1-arrest signaling"
Author(s):		Shi C; Kendall SC; Grote E; Kaminskyj S; Loewen MC;
Address:		"Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, SK, Canada"
Journal Title:		J Cell Biochem
Year:		2009
Volume:		107
Issue:		4
Page Number:		630 - 638
DOI:		10.1002/jcb.22129
ISSN/ISBN:		1097-4644 (Electronic) 0730-2312 (Linking)
Abstract:		"In Saccharomyces cerevisiae, mechanisms modulating the mating steps following cell cycle arrest are not well characterized. However, the N-terminal domain of Ste2p, a G protein-coupled pheromone receptor, was recently proposed to mediate events at this level. Toward deciphering receptor mechanisms associated with this mating functionality, scanning mutagenesis of targeted regions of the N-terminal domain has been completed. Characterization of ste2 yeast overexpressing Ste2p variants indicated that residues Ile 24 and Ile 29 as well as Pro 15 are critical in mediating mating efficiency. This activity was shown to be independent of Ste2p mediated G1 arrest signaling. Further analysis of Ile 24 and Ile 29 highlight the residues' solvent accessibility, as well as the importance of the hydrophobic nature of the sites, and in the case of Ile 24 the specific size and shape of the side chain. Mutation of these Ile's led to arrest of mating after cell contact, but before completion of cell wall degradation. We speculate that these extracellular residues mediate novel receptor interactions with ligand or proteins, leading to stimulation of alternate signaling effector pathways"
Keywords:		"*Cell Cycle G1 Phase Hydrophobic and Hydrophilic Interactions Ligands Mutagenesis, Site-Directed Receptors, Pheromone/genetics/*physiology Reproductive Physiological Phenomena Saccharomyces cerevisiae/cytology/*physiology *Signal Transduction Solvents;"
Notes:		"MedlineShi, Chunhua Kendall, Stephanie C Grote, Eric Kaminskyj, Susan Loewen, Michele C eng Research Support, Non-U.S. Gov't 2009/05/22 J Cell Biochem. 2009 Jul 1; 107(4):630-8. doi: 10.1002/jcb.22129"