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Insect Biochem Mol Biol

Title:		Antennal ionotropic receptors IR64a1 and IR64a2 of the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidate) collaboratively perceive habitat and host cues
Author(s):		Shan S; Wang SN; Song X; Khashaveh A; Lu ZY; Dhiloo KH; Li RJ; Gao XW; Zhang YJ;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; College of Plant Protection, China Agricultural University, Beijing, 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Institute of Plant and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing, 100097, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; College of Plant Protection, Agricultural University of Hebei, Baoding, 071000, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, 071000, China. Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam, 70060, Pakistan. College of Plant Protection, Agricultural University of Hebei, Baoding, 071000, China. College of Plant Protection, China Agricultural University, Beijing, 100193, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: yjzhang@ippcaas.cn"
Journal Title:		Insect Biochem Mol Biol
Year:		2019
Volume:		20190815
Issue:
Page Number:		103204 -
DOI:		10.1016/j.ibmb.2019.103204
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Linking)
Abstract:		"Ionotropic receptors (IRs), as a member of the conserved chemoreceptor families in the peripheral nervous system, play a critical role in the chemoreception of Drosophila. However, little is known about IRs in Hymenoptera insects. Here, we comprehensively characterized the gene structure, topological map and chemosensory roles of antennal IRs (MmedIRs) in the hymenopteran parasitoid wasp Microplitis mediator. We found that the IRs were conserved across various insect species. In the in situ hybridization assays, most IRs showed female antennae biased features, and there was no co-expression of the IRs and the olfactory receptor co-receptor (ORco). Moreover, three IR co-expressed complexes, IR75u-IR8a, IR64a1-IR8a and IR64a2-IR8a, were detected. Two genes with high similarity, IR64a1 and IR64a2, were located in distinct neurons but projected to the same sensillum. In two-electrode voltage-clamp recordings, IR64a1 was widely tuned to the chemicals from habitat cues released from host plants over long distances, whereas IR64a2 responded to a narrow range host cues and plant odors with low-volatility. Notably, IR64a2 was able to perceive Z9-14: Ald, a vital sex pheromone component that is released from Helicoverpa armigera, which is the preferred host of M. mediator. Furthermore, most ligands of IR64a1 and IR64a2 can trigger electrophysiological responses in female wasps. We propose that IR64a1 and IR64a2 collaboratively perceive habitat and host cues to assist parasitoids in efficiently seeking hosts"
Keywords:		"Animals Arthropod Antennae/*metabolism Female Male Receptors, Ionotropic Glutamate/genetics/*metabolism Wasps/genetics/*metabolism Xenopus Antennal ionotropic receptors Habitat cues Host cues Host-seeking Microplitis mediator Xenopus oocytes expression;"
Notes:		"MedlineShan, Shuang Wang, Shan-Ning Song, Xuan Khashaveh, Adel Lu, Zi-Yun Dhiloo, Khalid Hussain Li, Rui-Jun Gao, Xi-Wu Zhang, Yong-Jun eng Research Support, Non-U.S. Gov't England 2019/08/20 Insect Biochem Mol Biol. 2019 Nov; 114:103204. doi: 10.1016/j.ibmb.2019.103204. Epub 2019 Aug 15"