| Title: | Exploration of Potential Breath Biomarkers of Chronic Kidney Disease through Thermal Desorption-Gas Chromatography/Mass Spectrometry |
| Author(s): | Seong SH; Kim HS; Lee YM; Kim JS; Park S; Oh J; |
| Address: | "Mass Spectrometry & Advanced Instrumentation Group, Korea Basic Science Institute, Cheonju 28119, Republic of Korea. College of Pharmacy, Chungbuk National University, Cheongju 28644, Republic of Korea. ASTA Corporation, Research & Development Center, Suwon 16229, Republic of Korea. Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul 05355, Republic of Korea. Koscom Fund Services Corporation, Seoul 07330, Republic of Korea. Department of Internal Medicine, Kangdong Sacred Heart Hospital, Hallym University College of Medicine, Seoul 05355, Republic of Korea" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "Breath volatile organic compound (VOC) analysis is a non-invasive tool for assessing health status; the compositional profile of these compounds in the breath of patients with chronic kidney disease is believed to change with decreasing renal function. We aimed to identify breath VOCs for recognizing patients with chronic kidney disease. Using thermal desorption-gas chromatography/mass spectrometry, untargeted analysis of breath markers was performed using breath samples of healthy controls (n = 18) versus non-dialysis (n = 21) and hemodialysis (n = 12) patients with chronic kidney disease in this cross-sectional study. A total of 303 VOCs alongside 12 clinical variables were used to determine the breath VOC profile. Metabolomic analysis revealed that age, systolic blood pressure, and fifty-eight breath VOCs differed significantly between the chronic kidney disease group (non-dialysis + hemodialysis) and healthy controls. Thirty-six VOCs and two clinical variables that showed significant associations with chronic kidney disease in the univariate analysis were further analyzed. Different spectra of breath volatile organic compounds between the control and chronic kidney disease groups were obtained. A multivariate model incorporating age, 2-methyl-pentane, and cyclohexanone showed high performance (accuracy, 86%) in identifying patients with chronic kidney disease with odds ratios of 0.18 (95% CI, 0.07-2.49, p = 0.013); 2.10 (0.94-2.24, p = 0.025); and 2.31 (0.88-2.64, p = 0.008), respectively. Hence, this study showed that renal dysfunction induces a characteristic profile of breath VOCs that can be used as non-invasive potential biomarkers in screening tests for CKD" |
| Keywords: | breath markers chronic kidney disease hemodialysis mass spectrometry uremic toxins volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINESeong, Si-Hyun Kim, Hyun Sik Lee, Yong-Moon Kim, Jae-Seok Park, Sangwoo Oh, Jieun eng 2021RIS-001/Ministry of Education of Korea/ Switzerland 2023/07/29 Metabolites. 2023 Jul 11; 13(7):837. doi: 10.3390/metabo13070837" |
